Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8079
Title: Keratoconjunctivitis sicca of human T cell lymphotropic virus type 1 (HTLV-1) infected individuals is associated with high levels of HTLV-1 proviral load
Authors: Vargens, Cristina Castro Lima
Grassi, Maria Fernanda Rios
Boa-Sorte, Ney Cristian Amaral
Pinheiro, Regina Helena Rathsam
Gallazzi, Viviana Nilla Olavarria
Kruschewsky, Ramon de Almeida
Castro Filho, Bernardo Galvão
Affilliation: Instituto Brasileiro de Oftalmologia e Prevenção da Cegueira. IBOPC. Salvador, BA, Brasil.
Centro Integrativo e Multidisciplinar de HTLV. Escola Bahiana de Medicina e Saúde Pública. EBMSP. Salvador, BA, Brasil.
Centro Integrativo e Multidisciplinar de HTLV. Escola Bahiana de Medicina e Saúde Pública. EBMSP. Salvador, BA, Brasil.
Instituto Brasileiro de Oftalmologia e Prevenção da Cegueira. IBOPC. Salvador, BA, Brasil / Centro Integrativo e Multidisciplinar de HTLV. Escola Bahiana de Medicina e Saúde Pública. EBMSP. Salvador, BA, Brasil.
Centro Integrativo e Multidisciplinar de HTLV. Escola Bahiana de Medicina e Saúde Pública. EBMSP. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde .Pública. Salvador, BA, Brasil
Instituto Brasileiro de Oftalmologia e Prevenção da Cegueira. IBOPC. Salvador, BA, Brasil.
Centro Integrativo e Multidisciplinar de HTLV. Escola Bahiana de Medicina e Saúde Pública. EBMSP. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.
Abstract: BACKGROUND: A high HTLV-1 proviral load is found in HTLV-1-associated diseases, mainly HAM/TSP. However, the association between proviral load and keratoconjunctivitis sicca (KCS) has not been well established. AIM: To verify the association between KCS and HTLV-1 proviral load. STUDY DESIGN: 104 HTLV-1 infected patients (51 asymptomatic and 52 with HAM/TSP) from the HTLV reference center in Salvador, Brazil were followed from June 2008 to May 2010. Evaluation of tear secretion was performed by BUT (break-up time), Rose Bengal and Schirmer I tests. The diagnosis of KCS was based upon the presence of symptoms and when at least two of three tests were positive. HTLV-1 proviral load was determined using real-time PCR. RESULTS: The prevalence of KCS was 44.2%. KCS was more frequent among HAM/TSP patients (p = 0.022). Patients with KCS had higher proviral load (mean 134,672 ± 150,393copies/10(6) PBMC) than patients without the disease (mean 66,880 ± 109,525copies/10(6) PBMC) (p = 0.001). HTLV-1 proviral load>100,000copies/10(6) PBMC increased significantly the risk of developing KCS (OR = 4.05 and 95% CI = 1.40-11.76). After age>45 years and HAM/TSP status were excluded in stepway reward analysis, the variables PVL>100,000 (OR = 4.77 and 95% CI = 1.83-12.44) still remained statistically significant. CONCLUSION: HTLV-1 proviral loads are higher in patients with KCS and may represent a relevant biological marker of disease.
Keywords: HTLV-1
Ocular lesions
Keratoconjuntivitis sicca
Proviral load
DeCS: Infecções por HTLV-I/virologia
Vírus 1 Linfotrópico T Humano/fisiologia
Ceratoconjuntivite Seca/virologia
Provirus/fisiologia
Carga Viral
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Marcadores Biológicos
Brasil
Criança
DNA Viral/análise
Feminino
Infecções por HTLV-I/diagnóstico
Vírus 1 Linfotrópico T Humano/genética
Humanos
Ceratoconjuntivite Seca/diagnóstico
Masculino
Meia-Idade
Paraparesia Espástica Tropical/diagnóstico
Paraparesia Espástica Tropical/virologia
Issue Date: 2011
Publisher: Elsevier
Citation: VARGENS, C. C. L. et al. Keratoconjunctivitis sicca of human T cell lymphotropic virus type 1 (HTLV-1) infected individuals is associated with high levels of HTLV-1 proviral load. Journal of Clinical Virology, v. 52, n. 3, p. 177-180, 2011.
DOI: 10.1016/j.jcv.2011.07.016
ISSN: 1873-5967
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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