Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8087
Title: Genetic variability of human immunodeficiency virus-1 in Bahia state, Northeast, Brazil: high diversity of HIV genotypes.
Authors: Monteiro, Joana Paixão
Alcantara, Luiz Carlos Júnior
Oliveira, Tulio de
Oliveira, Antonio Marcos
Melo, Marco Antônio Gomes
Brites, Carlos
Castro Filho, Bernardo Galvão
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.
South African National Bioinformatics Institute. Cape Town, South Africa.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.
Universidade Federal da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.
Abstract: The HIV-1 genetic variability in Bahia state, Brazil, was investigated. DNA samples from 229 and 213 HIV-1-infected individuals were analyzed using the heteroduplex mobility assay (HMA) in gag and env fragments, respectively. One hundred seventy-five samples were characterized in both genes. Thirty-two subtype F and BF recombinant viruses were sequenced and analyzed by phylogenetic methods. The combination of HMA and sequencing results showed that seven different HIV-1 genotypes comprised this sample: 147 (84%) B/B, 4 (2.3%) F/F, 3 (1.7%) B/F, 1 (0.6%) F/B, 1 (0.6%) F/D, 1 (0.6%) BF/F, and 18 (10.3%) BF/B. A significant divergence was observed between these two techniques results (84.4%). This is explained by the low accuracy of the HMA for detecting recombinant viruses. These recombinants were unrelated to CRF12, while two sequences were related to CRF28 and CRF29. Nineteen BF mosaics shared the same gag breakpoint. In conclusion, the use of HMA may be inappropriate in regions where different subtypes are co-circulating. Subtype B is the most common genotype, however, an increased prevalence (13.1%) of different BF variants and a potentially new CRF suggest that recombination is occurring frequently in Bahia. These viruses were associated with women infected heterosexually. Finally, this study identified the presence of an F/D recombinant HIV-1 in Brazil.
Keywords: HIV-1
Epidemiology
Phylogeny
DeCS: Variação Genética
Infecções por HIV/epidemiologia
Infecções por HIV/virologia
HIV-1/classificação
HIV-1/genética
Adulto
Brasil/epidemiologia
Análise por Conglomerados
DNA Viral/genética
HIV-1/isolamento & purificação
HIV-1/isolamento & purificação
Feminino
Genótipo
Análise Heteroduplex
Humanos
Masculino
Dados de Sequência Molecular
Análise de Sequência de DNA
Filogenia
Homologia de Sequência
Produtos do Gene env do Vírus da Imunodeficiência Humana/genética
Issue Date: 2009
Publisher: Wiley-Liss, INC
Citation: MONTEIRO, J. P. et al. Genetic variability of human immunodeficiency virus-1 in Bahia state, Northeast, Brazil: high diversity of HIV genotypes. Journal of Medical Virology, v. 81, n. 3, p. 391–399, 2009.
DOI: 10.1002/jmv.21414
ISSN: 1096-9071
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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