Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8119
Title: Smoking-dependent and haplotype-specific effects of endothelial nitric oxide synthase gene polymorphisms on angiographically assessed coronary artery disease in Caucasian- and African-Brazilians.
Authors: Rios, Domingos Lázaro Souza
D’Onofrio, Lorenza O
Souza, Joares Kelton de Oliveira
Queiroz, Amália M
Maron, Luciana Raduy
Silva Neto, Naila
Carvalho, Heitor Ghissoni de
Santos Filho, Ademar
Castro Filho, Bernardo Galvão
Affilliation: Universidade Estadual do Sudoeste da Bahia. UESB. Laboratório de Genética Molecular. Departamento de Ciências Biológicas. Jequié, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Fundação Bahiana para o Desenvolvimento das Ciências. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Fundação Bahiana para o Desenvolvimento das Ciências. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Fundação Bahiana para o Desenvolvimento das Ciências. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Hospital Santa Izabel. Salvador, BA, Brasil
Hospital Santa Izabel. Salvador, BA,
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Fundação Bahiana para o Desenvolvimento das Ciências. Salvador, BA, Brasil
Abstract: The nitric oxide produced by endothelial nitric oxide synthase (eNOS) plays a pivotal role in protecting the arterial wall from damages and atherosclerosis. The T–786C, the 27-bp repeat in intron 4, and the E298D eNOS gene polymorphisms were studied in 715 Brazilian patients (447 Caucasian- and 268 African-Brazilians) who underwent coronary angiography. The −786C frequency was increased in coronary artery disease (CAD) cases with significant lesions (≥50% luminal obstruction) when compared with lesion-free controls; this difference was detected in smokers but not in nonsmokers, both in Caucasian- (p = 0.011) and African-Brazilians (p = 0.005). The interaction between−786C carriers and smoking was an independent CAD predictor (OR: 2.9, 95% CI: 1.4–5.9; p = 0.003) in multiple logistic regression. The 298D mutation frequency was also higher among CAD cases (p = 0.036) in African-Brazilian smokers, but this effect was not independent from other variables in the regression model. Though not associated with CAD, the 4-repeat allele combined with different T–786C alleles showed protective and susceptible effects in Caucasian-Brazilian smokers. The −786C/4-repeat/298E haplotype frequency was higher (p = 0.020), whereas −786T/4-repeat/298E was lower (p = 0.023) in these cases. These results showed a smoking-dependent effect of the T–786C eNOS polymorphism on CAD in both Caucasian- and African-Brazilians. Additionally, the haplotype analysis revealed different eNOS haplotypes associated with protection and susceptibility to the disease.
Keywords: Coronary artery disease
Endothelial nitric oxide synthase
Polymorphisms
African-Brazilians
Grupo com Ancestrais do Continente Africano/genética
Alelos
DeCS: Doença da Artéria Coronariana/enzimologia
Óxido Nítrico Sintase Tipo III/genética
Polimorfismo Genético
Adulto
Brasil
Estudos de Casos e Controles
Angiografia Coronária
Doença da Artéria Coronariana/etiologia
Grupo com Ancestrais do Continente Europeu/genética
Feminino
Frequência do Gene
Haplotipos
Heterozigoto
Homozigoto
Humanos
Íntrons
Masculino
Meia-Idade
Sequências Repetitivas do Ácido Nucleico
Hábito de Fumar/efeitos adversos
Issue Date: 2006
Publisher: Elsevier
Citation: RIOS, D. L. S. et al. Smoking-dependent and haplotype-specific effects of endothelial nitric oxide synthase gene polymorphisms on angiographically assessed coronary artery disease in Caucasian- and African-Brazilians. Atherosclerosis, v. 193, n. 1, p. 135–141, 2007.
DOI: 10.1016/j.atherosclerosis.2006.05.041
ISSN: 0021-9150
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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