Title:	Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
Authors:	Ferraro, Geraldo Argolo Mello, Marco Antônio Gomes Sutmöller, Frits Van Weyenbergh, Johan Jozef Rosa Maria Brazilian Network For HIV isolation and characterizationI Shindo, Nice Castro Filho, Bernardo Galvão Bou-Habib, Dumith Chequer
Affilliation:	Escola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / UNESCO. Ministério da Saude. CN/DST/AIDS. Salvador, BA, Brasil Escola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / UNESCO. Ministério da Saude. CN/DST/AIDS. Salvador, BA, Brasil Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunorregulação. Salvador, BA, Brasil Fundação Oswaldo Cruz. Salvador, BA, Brasil Escola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil Escola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Abstract:	The human immunodeficiency virus type 1 (HIV-1), the etiological agent of the acquired immunodeficiency syndrome (AIDS), shows a variety of biological properties, which may constitute an obstacle to development of effective vaccines or antiretroviral therapy. To characterize Brazilian strains of HIV-1, we studied 24 viruses isolated from blood samples of HIV-1-positive patients from different regions of the country. To examine the cell tropism and the virus ability to form syncytia, primary macrophages and the CD41 T cell line MT-2 were infected with these viruses. We found that 22 isolates replicated well in macrophages (macrophage-tropic isolates), 2 infected only MT-2 cells (T cell line tropic variants), while 6 of them grew in both cells. We found 8 syncytium-inducing (SI) and 16 non-SI (NSI) isolates. Continuous cultures of 18 isolates were established in the CCR51 /CXCR41 cell line PM-1, and SI/NSI features of these viruses were confirmed by cell fusion assay with uninfected CD41 T cell lines (PM-1, MT-2, H9, and SUP-T1). The coreceptor usage of 18 isolates was investigated by infecting U87 cells transfected with CD4 and chemokine receptors, and we found that 11 isolates infected only CCR51 cells, 3 only CXCR41 cells, whereas 4 used both coreceptors. We also observed that X4 isolates were more sensitive to neutralization by dextran sulfate than R5 or R5X4 viruses. Our findings show that the Brazilian isolates are phenotypically similar to those prevalent in other regions, which could mean that therapeutic strategies based on HIV-1 phenotypic properties would be efficient in Brazil, as in other countries.
DeCS:	Infecções por HIV/virologia HIV-1/isolamento & purificação HIV-1/metabolismo Receptores CCR5/metabolismo Brasil/epidemiologia Linhagem Celular Sulfato de Dextrana/farmacologia Células Gigantes/efeitos de drogas Infecções por HIV/epidemiologia HIV-1/efeitos de drogas HIV-1/genética Humanos Macrófagos/efeitos de drogas Macrófagos/virologia Especificidade por Substrato
Issue Date:	2001
Publisher:	Mary Ann Liebert, Inc
Citation:	FERRARO, G. A. et al. Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil. AIDS Research and Human Retroviruses, v. 17, n. 13, p. 1241-1247, 2001.
ISSN:	0889-2229
Copyright:	open access
Appears in Collections:	BA - IGM - Artigos de Periódicos

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