Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8214
Title: Identification of human immunodeficiency virus type 1 envelope genes recombinant between subtypes B and F in two epidemiologically linked individuals from Brazil.
Authors: Sabino, Ester Cerdeira
Shpaer, Eugene G
Morgado, Mariza Gonçalves
Korber, Bette T. M
Diaz, Ricardo Sobhie
Bongertz, Vera
Cavalcante, Solange
Castro Filho, Bernardo Galvão
Mullins, James I
Mayer, Allen
Affilliation: Irwin Memorial Blood Centers. San Francisco, California / Instituto Adolfo Lutz. Laboratorio de Retrovirus. São Paulo, SP, Brasil
University School of Medicine. Department of Microbiology and Immunology. Stanford, California
Irwin Memorial Blood Centers. San Francisco, California / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil
Los Alamos National Laboratory. Los Alamos, New Mexico / Santa Fe Institute. Santa Fe, New Mexico
Irwin Memorial Blood Centers. San Francisco, California / Escola Paulista de Medicina. DIPA. Sao Paulo, SP, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Púbica. Salvador, BA, Brasil
University School of Medicine. Department of Microbiology and Immunology. Stanford, California
Irwin Memorial Blood Centers. San Francisco, California
Abstract: Sequence analysis of a human immunodeficiency virus type 1 env gene PCR amplified from a Brazilian woman's peripheral blood mononuclear cell DNA (sample RJIO1) showed that it was likely to have been derived from a double recombination event between human immunodeficiency virus type 1 subtypes B and F. The major portion of the gp120 coding sequence belonged to the B lineage, but a segment of the C2 to V3 region (approximately 135 nucleotides) clearly associated with sequences of the F lineage. The subtype F-like segment had 15 noncontiguous signature nucleotides in common with Brazilian subtype F sequences that were not found, or were rare, in subtype B sequences. In contrast, this same segment had only 3 signature nucleotides shared with subtype B sequences and not present in the Brazilian subtype F sequences. Phylogenetic analysis, amino acid signature pattern analysis, and the pattern of synonymous mutations all supported the hypothesis of a recombinational origin of the RJIO1 sequence. Related recombinant genes were also detected in peripheral blood mononuclear cell DNA obtained from the woman's recent sexual partner, indicating that the recombination event probably occurred at some previous time in the chain of virus transmission. Divergent viral sequences in the V3 region were found in the male sexual partner, while a relatively homogeneous viral population was detected in the woman, consistent with her recent infection
DeCS: Síndrome de Imunodeficiência Adquirida/microbiologia
Produtos do Gene env/genética
Genes env
Proteína gp120 do Envelope de HIV/genética
HIV-1/genética
Filogenia
Recombinação Genética
Síndrome de Imunodeficiência Adquirida/epidemiologia
Sequência de Aminoácidos
Sequência de Bases
Brasil/epidemiologia
Primers do DNA
Feminino
Produtos do Gene env/química
HIV-1/classificação
Humanos
Dados de Sequência Molecular
Reação em Cadeia da Polimerase/métodos
Homologia de Sequência de Aminoácidos
Issue Date: 1994
Publisher: American Society for Microbiology
Citation: SABINO, E. C. et al. Identification of human immunodeficiency virus type 1 envelope genes recombinant between subtypes B and F in two epidemiologically linked individuals from Brazil. Journal of Virology, v. 68, n. 10, p. 6340-6346, 1994.
ISSN: 0022-538X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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