Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8295
Title: Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model.
Authors: Baqueiro, Tiana
Russo, Momtchilo
Silva, Virgínia Maria Goes da
Meirelles, Thayna
Oliveira, Pablo Rafael Silveira
Gomes, Eliane
Barboza, Renato
Lima, Ana Tereza Cerqueira
Figueiredo, Camila Alexandrina Viana
Pontes-de-Carvalho, Lain Carlos
Neves, Neuza Maria Alcântara
Affilliation: Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Universidade Federal da Bahia. Nucleo de Tecnologia em Saude. Instituto Multidisciplinar em Saude. Vitoria da Conquista, BA, Brasil
Universidade de São Paulo. São Paulo. Instituto de Ciencias Biomedicas. Departamento de Imunologia. São Paulo, SP, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade de São Paulo. São Paulo. Instituto de Ciencias Biomedicas. Departamento de Imunologia. São Paulo, SP, Brasil
Universidade de São Paulo. São Paulo. Instituto de Ciencias Biomedicas. Departamento de Imunologia. São Paulo, SP, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Universidade Federal da Bahia. Nucleo de Tecnologia em Saude. Instituto Multidisciplinar em Saude. Vitoria da Conquista, BA, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Universidade Federal da Bahia. Nucleo de Tecnologia em Saude. Instituto Multidisciplinar em Saude. Vitoria da Conquista, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil
Abstract: BACKGROUND: The dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Although a mouse model for B. tropicalis extract (BtE)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal, the molecular composition of the allergen and the experimental protocol. OBJECTIVES: This work had two objectives. The first was to study the anti-B. tropicalis allergic responses in different mouse strains using a short-term model of respiratory allergy to BtE. This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. The second objective was to investigate whether the best responder mouse strain could be used in an experimental model of allergy employing relatively low BtE doses. METHODS: Groups of mice of four different syngeneic strains were sensitized subcutaneously with 100 microg of BtE on days 0 and 7 and challenged four times intranasally, at days 8, 10, 12, and 14, with 10 microg of BtE. A/J mice, that were the best responders to BtE sensitization, were used to compare the B. tropicalis-specific asthma experimental model with the conventional experimental model of ovalbumin (OVA)-specific asthma. A/J mice were also sensitized with a lower dose of BtE. RESULTS: Mice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. Finally, immunization with a relatively low BtE dose (10 microg per subcutaneous injection per mouse) was able to sensitize A/J mice, which were the best responders to high-dose BtE immunization, for the development of allergy-associated immune and lung inflammatory responses. CONCLUSIONS: The described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE.
DeCS: Alérgenos/administração & dosagem
Asma/imunologia
Pyroglyphidae/imunologia
Administração Intranasal
Animais
Antígenos de Plantas
Asma/genética
Hiper-Reatividade Brônquica/imunologia
Líquido da Lavagem Broncoalveolar/citologia
Citocinas/metabolismo
Modelos Animais de Doenças
Relação Dose-Resposta Imunológica
Imunoglobulina E/sangue
Injeções Subcutâneas
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos BALB C
Ovalbumina
Eosinofilia Pulmonar/imunologia
Ratos
Ratos Wistar
Especificidade da Espécie
Fatores de Tempo
Issue Date: 2010
Publisher: BioMed Central
Citation: BAQUEIRO, T. et al. Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. Respiratory Research, v. 11, p.51, 2010.
DOI: 10.1186/1465-9921-11-51
ISSN: 1465-993X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Baqueiro Tiana Respiratory allergy to Blomia.....pdf783.9 kBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.