| Author | Santos, Lenita Ramires dos | |
| Author | Melo, Stella Maria Barrouin | |
| Author | Chang, Yung-Fu | |
| Author | Olsen, John | |
| Author | McDonough, Sean P | |
| Author | Quimby, Fred | |
| Author | dosSantos, Washington Luis Conrado | |
| Author | Pontes-de-Carvalho, Lain Carlos | |
| Author | Oliveira, Geraldo Gileno de Sá | |
| Access date | 2014-09-08T12:00:17Z | |
| Available date | 2014-09-08T12:00:17Z | |
| Document date | 2004 | |
| Citation | SANTOS, enita Ramires dos et al. Recombinant single-chain canine interleukin 12 induces interferon gamma mRNA expression in peripheral blood mononuclear cells of dogs with visceral leishmaniasis. Veterinary Immunology Immunopathology, v. 98, n. 1-2, p. 43-48, 2004. | pt_BR |
| ISSN | 0165-2427 | |
| URI | https://www.arca.fiocruz.br/handle/icict/8309 | |
| Language | eng | pt_BR |
| Publisher | Elsevier B.V | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Recombinant single-chain canine interleukin 12 induces interferon gamma mRNA expression in peripheral blood mononuclear cells of dogs with visceral leishmaniasis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vetimm.2003.10.006 | |
| Abstract | Canine visceral leishmaniasis poses important concerns for public health and veterinary medicine in many areas of the world.
Resistance to it seems to be associated with cellular specific immune responses of the so-called Th1 type. Interleukin-12 (IL-12)
is one of the most potent inducers of Th1 type of immune responses to co-administered antigens. Herein, the cloning of canine
IL-12, as a single-chain fusion protein (sccaIL-12), and its expression in biologically active form in COS-7 cells is reported.
Supernatants from these cells stimulated the expression of comparable amounts of interferon gamma mRNA in peripheral blood
mononuclear cells from dogs with natural visceral leishmaniasis. In addition, after stimulation with sccaIL-12, there was no
difference between interferon gamma mRNA expressions in peripheral blood mononuclear cells of dogs with visceral
leishmaniasis and from normal healthy control animals. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Cornell University. College of Veterinary Medicine. Department of Population Medicine and Diagnostic Sciences. Ithaca, NY, USA. | pt_BR |
| Affilliation | Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA. | pt_BR |
| Affilliation | Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA. | pt_BR |
| Affilliation | Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Subject | Canine recombinant IL-12 | pt_BR |
| Subject | Visceral leishmaniasis | pt_BR |
| Subject | Gamma interferon expression | pt_BR |
| DeCS | Doenças do Cão/quimioterapia | pt_BR |
| DeCS | Indutores de Interferon/farmacologia | pt_BR |
| DeCS | Interleucina-12/farmacologia | pt_BR |
| DeCS | Interferon gama/genética | pt_BR |
| DeCS | Leishmaniose Visceral/veterinária | pt_BR |
| DeCS | RNA Mensageiro/genética | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Sequência de Bases | pt_BR |
| DeCS | Clonagem Molecular | pt_BR |
| DeCS | Doenças do Cão/genética | pt_BR |
| DeCS | Cães | pt_BR |
| DeCS | Expressão Gênica | pt_BR |
| DeCS | Indutores de Interferon/química | pt_BR |
| DeCS | Interleucina-12/química | pt_BR |
| DeCS | Leishmaniose Visceral/quimioterapia | pt_BR |
| DeCS | Leucócitos Mononucleares/efeitos de drogas | pt_BR |
| DeCS | Proteínas Recombinantes/química | pt_BR |
| DeCS | Proteínas Recombinantes/farmacologia | pt_BR |
