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RECOMBINANT SINGLE-CHAIN CANINE INTERLEUKIN 12 INDUCES INTERFERON GAMMA MRNA EXPRESSION IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF DOGS WITH VISCERAL LEISHMANIASIS
Indutores de Interferon/farmacologia
Interleucina-12/farmacologia
Interferon gama/genética
Leishmaniose Visceral/veterinária
RNA Mensageiro/genética
Animais
Sequência de Bases
Clonagem Molecular
Doenças do Cão/genética
Cães
Expressão Gênica
Indutores de Interferon/química
Interleucina-12/química
Leishmaniose Visceral/quimioterapia
Leucócitos Mononucleares/efeitos de drogas
Proteínas Recombinantes/química
Proteínas Recombinantes/farmacologia
Author
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Cornell University. College of Veterinary Medicine. Department of Population Medicine and Diagnostic Sciences. Ithaca, NY, USA.
Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA.
Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA.
Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Cornell University. College of Veterinary Medicine. Department of Population Medicine and Diagnostic Sciences. Ithaca, NY, USA.
Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA.
Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA.
Cornell University. College of Veterinary Medicine. Department of Biomedical Sciences. Ithaca, NY, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Abstract
Canine visceral leishmaniasis poses important concerns for public health and veterinary medicine in many areas of the world.
Resistance to it seems to be associated with cellular specific immune responses of the so-called Th1 type. Interleukin-12 (IL-12)
is one of the most potent inducers of Th1 type of immune responses to co-administered antigens. Herein, the cloning of canine
IL-12, as a single-chain fusion protein (sccaIL-12), and its expression in biologically active form in COS-7 cells is reported.
Supernatants from these cells stimulated the expression of comparable amounts of interferon gamma mRNA in peripheral blood
mononuclear cells from dogs with natural visceral leishmaniasis. In addition, after stimulation with sccaIL-12, there was no
difference between interferon gamma mRNA expressions in peripheral blood mononuclear cells of dogs with visceral
leishmaniasis and from normal healthy control animals.
DeCS
Doenças do Cão/quimioterapiaIndutores de Interferon/farmacologia
Interleucina-12/farmacologia
Interferon gama/genética
Leishmaniose Visceral/veterinária
RNA Mensageiro/genética
Animais
Sequência de Bases
Clonagem Molecular
Doenças do Cão/genética
Cães
Expressão Gênica
Indutores de Interferon/química
Interleucina-12/química
Leishmaniose Visceral/quimioterapia
Leucócitos Mononucleares/efeitos de drogas
Proteínas Recombinantes/química
Proteínas Recombinantes/farmacologia
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