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Title: Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
Authors: Neves, Neuza Maria Alcântara
Pontes-de-Carvalho, Lain Carlos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat6rio de Imunologia Molecular e Celular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Púbica. Salvador, BA, Brasil
Abstract: Parasite-derived trans-sialidase (TS) activity was demonstrated in the serum and blood of Trypanosoma cruzi-infected mice. Serum TS activity levels correlated well with parasitemia in BALB/c and Swiss mice during the initial stages of the infection. However, in later stages the TS activity levels decreased despite increasing parasitemia. This coincided with the appearance of circulating TS antibodies. On the other hand, there was always a good correlation between TS activity and parasitemia in athymic nude mice. Sera from mice with high parasitemia and low TS activity inhibited TS activity in vitro. The inhibition was also observed with purified serum IgG, and it was absorbed by staphylococcal protein A, indicating that it was caused by anti-TS IgG antibodies. These antibodies inhibited the enzymatic activity of insolubilized TS, indicating that they act by interfering with the catalytic site rather than by aggregating the enzyme. The presence of inhibitory antibodies, however, did not prevent the progression of parasitemia in BALB/c mice.
DeCS: Anticorpos Antiprotozoários/sangue
Doença de Chagas /sangue
Glicoproteínas /sangue
Neuraminidase /sangue
Trypanosoma cruzi /enzimologia
Western Blotting
Doença de Chagas /enzimologia
Eletroforese em Gel de Poliacrilamida
Glicoproteínas /antagonistas & inibidores
Imunoglobulina G /isolamento & purificação
Camundongos Endogâmicos BALB C
Neuraminidase /antagonistas & inibidores
Parasitemia /enzimologia
Fatores de Tempo
Trypanosoma cruzi /imunologia
Issue Date: 1995
Publisher: Springer Verlag
Citation: ALCÂNTARA-NEVES, N. M.; PONTES-DE-CARVALHO, L. C. Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice. Parasitology Research, v. 81, n. 7, p. 560-564, 1995.
ISSN: 0932-0113
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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