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https://www.arca.fiocruz.br/handle/icict/8405
PRIMARY MUSCLE DISEASE: DEFINITION OF A 25-KDA POLYPEPTIDE MYOPATHIC SPECIFIC CHAGAS ANTIGEN
Cardiomiopatia Chagásica/imunologia
Ensaio de Imunoadsorção Enzimática
Epitopos
Reações Falso-Positivas
Humanos
Imunoglobulina G/análise
Microssomos/imunologia
Miocárdio/citologia
Sarcolema/imunologia
Retículo Sarcoplasmático/imunologia
Trypanosoma cruzi/imunologia
Author
Affilliation
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Merck Sharp and Dohme Research Laboratories. Department of Immunology and Inflammation Research. Rahxaay. New Jersey
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Cornell University Medical College. Departments of Pathology and Medicine. New York, NY
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Merck Sharp and Dohme Research Laboratories. Department of Immunology and Inflammation Research. Rahxaay. New Jersey
Abstract
The sera from patients with primary heart and skeletal muscle diseases, hospitalized patients without intrinsic muscle disease from an area endemic for Trypanosoma cruzi infections, and normal subjects (N = 693) were studied for the presence of immunoglobulin G (IgG) antisarcolemma activity using serologic methods. The prevalence of elevated serum IgG antisarcolemma activity from patients with chronic Chagas' cardiomyopathy, idiopathic cardiomyopathy, polymyositis, and Duchenne muscular dystrophy was 58.9 +/- 10.4% (N = 101) (P less than 0.001 when compared to normal subjects). Two of twelve (16.7%) patients with acute T. cruzi infection and parasitemia developed elevated antisarcolemma titers, and 9/46 (19.6%) patients with chronic T. cruzi infection without evidence of cardiomyopathy yielded high antisarcolemma titers. On the other hand, patients with chronic T. cruzi infection with advanced cardiomyopathy yielded high antisarcolemma titers in 35/74 (47.3%) (P less than 0.001 when compared to normal subjects). Radioimmunoprecipitation showed a circulating antibody to a 25-kDa T. cruzi polypeptide (P25) in 16/17 (94.1%) patients with advanced cardiomyopathy and T. cruzi infection. No such antibody was shown in 12 asymptomatic subjects with chronic T. cruzi infection.
DeCS
Anticorpos/análiseCardiomiopatia Chagásica/imunologia
Ensaio de Imunoadsorção Enzimática
Epitopos
Reações Falso-Positivas
Humanos
Imunoglobulina G/análise
Microssomos/imunologia
Miocárdio/citologia
Sarcolema/imunologia
Retículo Sarcoplasmático/imunologia
Trypanosoma cruzi/imunologia
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