Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8409
Title: Evaluation of immune responses and analysis of the effect of vaccination of the Leishmania major recombinant ribosomal proteins L3 or L5 in two different murine models of cutaneous leishmaniasis.
Authors: Garcia, Laura Ramírez
Santos, Diego Moura
Souza, Ana Paula Almeida de
Coelho, Eduardo Antonio Ferraz
Barral, Aldina Maria Prado
Alonso, Carlos
Escutia, Marta R
Bonay, Pedro
Oliveira, Camila Indiani de
Alvarez, Manuel Soto
Affilliation: Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. CSIC-UAM. Departamento de Biología Molecular. Madrid, Spain
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Minas Gerais. Faculdade de Medicina e Departamento de Patologia Clínica. COLTEC. Programa de Pós-Graduação em Ciências Saúde. I Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. CSIC-UAM. Departamento de Biología Molecular. Madrid, Spain
Research & Development Department. Laboratorios LETI S.L.u. Madrid, Spain
Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. CSIC-UAM. Departamento de Biología Molecular. Madrid, Spain
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. CSIC-UAM. Departamento de Biología Molecular. Madrid, Spain
Abstract: Four new antigenic proteins located in Leishmania ribosomes have been characterized: S4, S6, L3 and L5. Recombinant versions of the four ribosomal proteins from Leishmania major were recognized by sera from human and canine patients suffering different clinical forms of leishmaniasis. The prophylactic properties of these proteins were first studied in the experimental model of cutaneous leishmaniasis caused by L. major inoculation into BALB/c mice. The administration of two of them, LmL3 or LmL5 combined with CpG-oligodeoxynucleotides (CpG-ODN) was able to protect BALB/c mice against L. major infection. Vaccinated mice showed smaller lesions and parasite burden compared to mice inoculated with vaccine diluent or vaccine adjuvant. Protection was correlated with an antigen-specific increased production of IFN-γ paralleled by a decrease of the antigen-specific IL-10 mediated response in protected mice relative to non-protected controls. Further, it was demonstrated that BALB/c mice vaccinated with recombinant LmL3 or LmL5 plus CpG-ODN were also protected against the development of cutaneous lesions following inoculation of L. braziliensis. Together, data presented here indicate that LmL3 or LmL5 ribosomal proteins combined with Th1 inducing adjuvants, may be relevant components of a vaccine against cutaneous leishmaniasis caused by distinct species.
Keywords: Leishmania
BALB/c mice
Th1/Th2 Immune responses
Recombinant ribosomal proteins
Vaccines
DeCS: Leishmania major/imunologia
Vacinas contra Leishmaniose/imunologia
Leishmaniose Cutânea/prevenção & controle
Proteínas Ribossômicas/imunologia
Adjuvantes Imunológicos/administração & dosagem
Animais
Modelos Animais de Doenças
Feminino
Interferon gama/secreção
Leishmania braziliensis/imunologia
Vacinas contra Leishmaniose/administração & dosagem
Leishmaniose Cutânea/imunologia
Leucócitos Mononucleares/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Oligodesoxirribonucleotídeos/administração & dosagem
Proteínas Recombinantes/genética
Vacinas Sintéticas/administração & dosagem
Issue Date: 2013
Publisher: Elsevier Ltd
Citation: GARCIA, L. R. et al. Evaluation of immune responses and analysis of the effect of vaccination of the Leishmania major recombinant ribosomal proteins L3 or L5 in two different murine models of cutaneous leishmaniasis. Vaccine, v. 31, n. 9, p. 1312-1319, 2013.
DOI: 10.1016/j.vaccine.2012.12.071
ISSN: 1873-2518
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Ramirez L Evaluation....pdf920.34 kBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.