Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8552
Title: Anti-inflammatory effects of carvacrol: evidence for a key role of interleukin-10.
Authors: Lima, Milena da Silva
Quintans Junior, Lucindo José
Santana, Wagno Alcântara
Kaneto, Carla Martins
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
Affilliation: Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil
Universidade Federal de Sergipe. Departamento de Fisiologia. São Cristóvão, SE, Brasil
Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Carvacrol,aphenolicmonoterpene,hasbeenreportedtopossessanti-inflammatoryproperties. However,themechanismsinvolvedinitspharmacologicalpropertiesarecurrentlynotwellunder- stood. Inthepresentstudy,thecontributionofcytokinemodulationtotheanti-inflammatoryeffectsof carvacrolwasinvestigatedinaclassicalinflammationmodel:thecompleteFreund’sadjuvant(CFA)- inducedpawinflammationinmice.Thepawedemawasmeasuredusingaplesthismometer.Pawtissue was removed2haftertheinflammatorystimulustodeterminethelevelsofprostaglandinE2 (PGE2) by enzyme immunoassay,thelevelsofinterleukin-1 b (IL-1b), tumornecrosisfactor-a (TNF-a), and interleukin-10(IL-10)byELISAorthemRNAexpressionofcyclooxygenase-2(COX-2),IL-1b, TNF-a, and IL-10 byreal-timePCR.Administrationofcarvacrolproducedanti-inflammatoryeffectsagainstCFA- inducedinflammationinmice.Treatmentofmicewithcarvacrolat50and100mg/kgattenuatedthe paw edemaandreducedtheIL-1b and PGE2, butnotTNF-a, locallevels.Similarly,carvacrol(100mg/kg) reducedtheCOX-2andIL-1b mRNA expression.ThelevelsofIL-10,ananti-inflammatorycytokine,and the IL-10mRNAexpressionintheinflamedpawwereenhancedbycarvacrol.Inaddition,thetreatment with carvacroldidnotreducetheCFA-inducedpawedemainIL-10knockoutmice.Thepresentresults suggestthatcarvacrolcausesanti-inflammatoryeffectsbyreducingtheproductionofinflammatory mediators,suchasIL-1b and prostanoids,possiblythroughtheinductionofIL-10release.
Keywords: Carvacrol
Anti-inflammatory
Cytokines
Interleukin-10
PGE2
DeCS: Anti-Inflamatórios/farmacologia
Inflamação/quimioterapia
Interleucina-10/metabolismo
Monoterpenos/farmacologia
Animais
Anti-Inflamatórios/administração & dosagem
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Edema/quimioterapia
Regulação da Expressão Gênica/efeitos de drogas
Mediadores da Inflamação/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Monoterpenos/administração & dosagem
Reação em Cadeia da Polimerase em Tempo Real
Issue Date: 2013
Publisher: Elsevier B.V
Citation: LIMA, M. S. et al. Anti-inflammatory effects of carvacrol: evidence for a key role of interleukin-10. European Journal of Pharmacology, v. 699, n. 1-3, p. 112-117, 2013.
DOI: 10.1016/j.ejphar.2012.11.040
ISSN: 1879-0712
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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