Author | Ferraz, Rosana Paula Cruz | |
Author | Bomfim, Diogo S | |
Author | Carvalho, Nanashara Coelho de | |
Author | Soares, Milena Botelho Pereira | |
Author | Silva, Thanany Brasil da | |
Author | Machado, Wedna de Jesus | |
Author | Prata, Ana Paula do Nascimento | |
Author | Costa, Emmanoel Vilaça | |
Author | Moraes, Valéria Regina de Souza | |
Author | Nogueira, Paulo Cesar de Lima | |
Author | Bezerra, Daniel Pereira | |
Access date | 2014-10-08T18:53:10Z | |
Available date | 2014-10-08T18:53:10Z | |
Document date | 2013 | |
Citation | FERRAZ, R. P. C. et al. Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae). Phytomedicine, v. 20, n. 7, p. 615-621, 2013. | pt_BR |
ISSN | 1618-095X | |
URI | https://www.arca.fiocruz.br/handle/icict/8553 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae). | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.phymed.2013.01.015 | |
Abstract | Medicinal plants are one of the most important sources of drugs used in the pharmaceutical industry.
Among traditional medicinal plants, Lippia gracilis Schauer (Verbenaceae) had been used for several
medicinal purposes in Brazilian northeastern. In this study, leaf essential oil (EO) of L. gracilis was prepared
using hydrodistillation. Followed by GC–MS analysis, its composition was characterized by the
presence of thymol (55.50%), as major constituent. The effects of EO on cell proliferation and apoptosis
induction were investigated in HepG2 cells. Furthermore, mice bearing Sarcoma 180 tumor cells were
used to confirm its in vivo effectiveness. EO and its constituents (thymol, p-cymene, -terpinene and
myrcene) displayed cytotoxicity to different tumor cell lines. EO treatment caused G1 arrest in HepG2
cells accompanied by the induction of DNA fragmentation without affecting cell membrane integrity.
Cell morphology consistent with apoptosis and a remarkable activation of caspase-3 were also observed,
suggesting induction of caspase-dependent apoptotic cell death. In vivo antitumor study showed tumor
growth inhibition rates of 38.5–41.9%. In conclusion, the tested essential oil of L. gracilis leaves, which
has thymol as its major constituent, possesses significant in vitro and in vivo antitumor activity. These
data suggest that leaf essential oil of L. gracilis is a potential medicinal resource. | pt_BR |
Affilliation | Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Biology. São Cristóvão, SE, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Biology. São Cristóvão, SE, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brasil | pt_BR |
Subject | Lippia gracilis | pt_BR |
Subject | Essential oil | pt_BR |
Subject | Cytotoxicity | pt_BR |
Subject | Apoptosis | pt_BR |
Subject | Antitumor | pt_BR |
DeCS | Antineoplásicos Fitogênicos/farmacologia | pt_BR |
DeCS | Lippia/química | pt_BR |
DeCS | Óleos Voláteis/farmacologia | pt_BR |
DeCS | Alcenos/farmacologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Brasil | pt_BR |
DeCS | Linhagem Celular Tumoral/efeitos de drogas | pt_BR |
DeCS | Membrana Celular/efeitos de drogas | pt_BR |
DeCS | Cromatografia Gasosa-Espectrometria de Massas | pt_BR |
DeCS | Células Hep G2/efeitos de drogas | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Monoterpenos/farmacologia | pt_BR |
DeCS | Óleos Voláteis/análise | pt_BR |
DeCS | Folhas de Planta/química | pt_BR |
DeCS | Plantas Medicinais/química | pt_BR |