Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8553
Title: Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
Authors: Ferraz, Rosana Paula Cruz
Bomfim, Diogo S
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Silva, Thanany Brasil da
Machado, Wedna de Jesus
Prata, Ana Paula do Nascimento
Costa, Emmanoel Vilaça
Moraes, Valéria Regina de Souza
Nogueira, Paulo Cesar de Lima
Bezerra, Daniel Pereira
Affilliation: Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brasil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brasil
Federal University of Sergipe. Department of Biology. São Cristóvão, SE, Brasil
Federal University of Sergipe. Department of Biology. São Cristóvão, SE, Brasil
Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brasil
Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brasil
Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brasil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brasil
Abstract: Medicinal plants are one of the most important sources of drugs used in the pharmaceutical industry. Among traditional medicinal plants, Lippia gracilis Schauer (Verbenaceae) had been used for several medicinal purposes in Brazilian northeastern. In this study, leaf essential oil (EO) of L. gracilis was prepared using hydrodistillation. Followed by GC–MS analysis, its composition was characterized by the presence of thymol (55.50%), as major constituent. The effects of EO on cell proliferation and apoptosis induction were investigated in HepG2 cells. Furthermore, mice bearing Sarcoma 180 tumor cells were used to confirm its in vivo effectiveness. EO and its constituents (thymol, p-cymene, -terpinene and myrcene) displayed cytotoxicity to different tumor cell lines. EO treatment caused G1 arrest in HepG2 cells accompanied by the induction of DNA fragmentation without affecting cell membrane integrity. Cell morphology consistent with apoptosis and a remarkable activation of caspase-3 were also observed, suggesting induction of caspase-dependent apoptotic cell death. In vivo antitumor study showed tumor growth inhibition rates of 38.5–41.9%. In conclusion, the tested essential oil of L. gracilis leaves, which has thymol as its major constituent, possesses significant in vitro and in vivo antitumor activity. These data suggest that leaf essential oil of L. gracilis is a potential medicinal resource.
Keywords: Lippia gracilis
Essential oil
Cytotoxicity
Apoptosis
Antitumor
DeCS: Antineoplásicos Fitogênicos/farmacologia
Lippia/química
Óleos Voláteis/farmacologia
Alcenos/farmacologia
Animais
Brasil
Linhagem Celular Tumoral/efeitos de drogas
Membrana Celular/efeitos de drogas
Cromatografia Gasosa-Espectrometria de Massas
Células Hep G2/efeitos de drogas
Humanos
Masculino
Camundongos
Monoterpenos/farmacologia
Óleos Voláteis/análise
Folhas de Planta/química
Plantas Medicinais/química
Issue Date: 2013
Publisher: Elsevier
Citation: FERRAZ, R. P. C. et al. Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae). Phytomedicine, v. 20, n. 7, p. 615-621, 2013.
DOI: 10.1016/j.phymed.2013.01.015
ISSN: 1618-095X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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