Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8566
Title: Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
Authors: Larocca, Ticiana Ferreira
Freitas Souza, Bruno Solano de
Silva, Cristina Aragão
Kaneto, Carla Martins
Alcantara, Adriano Costa de
Azevedo, Carine Machado
Castro, Murilo Fagundes de
Macambira, Simone Garcia
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Abstract: BACKGROUND: Chagas disease, caused by the protozoan Trypanosoma cruzi, is a major cause of heart failure in Latin America. Tissue therapy has been investigated as a possible therapeutic option for patients with cardiovascular disease. OBJECTIVE: This study evaluated the effects of therapy with mesenchymal stem cells in an experimental model of chronic Chagasic cardiomyopathy. METHODS: C57BL/6 mice were infected with 1000 trypomastigotes from the Colombian strain of T. cruzi and, after six months of infection, were treated with mesenchymal human stem cells from adipose tissue (STAT) or with Dulbecco/Vogt modified Eagle's minimal essential medium - DMEM (control). The treated group received two intraperitoneal injections of STAT (1x10(6) cells/dose), with a month interval between the two doses. Before and after the first and second months of treatment, the chagasic and normal control animals underwent cardiopulmonary exercise testing and electrocardiography. All animals were sacrificed under anesthesia after two months of treatment for histopathological analysis of the heart. RESULTS: No improvement was observed in arrhythmias and cardiovascular function in the group of animals treated with STAT; however, sections of mice hearts in this group revealed a significant reduction in the number of inflammatory cells (p<0.0001) and areas of fibrosis (p<0.01) in comparison with chagasic animals treated with DMEM. CONCLUSION: Thus, it is concluded that administration of intraperitoneal STAT can reduce inflammation and fibrosis in the heart of mice chronically infected with T. cruzi; however, there were no effects on the cardiac function two months after transplantation.
Keywords: Chagas Cardiomyopathy / therapy
Stem Cells
Tissue Therapy
Adipose Tissue
DeCS: Tecido Adiposo/citologia
Cardiomiopatia Chagásica/cirurgia
Transplante de Células-Tronco Mesenquimais
Trypanosoma cruzi
Análise de Variância
Animais
Arritmias Cardíacas/metabolismo
Cardiomiopatia Chagásica/patologia
Cardiomiopatia Chagásica/fisiopatologia
Modelos Animais de Doenças
Fibrose
Inflamação/metabolismo
Injeções Intraperitoneais
Camundongos
Camundongos Endogâmicos C57BL
Condicionamento Físico Animal/métodos
Distribuição Aleatória
Issue Date: 2013
Publisher: Sociedade Brasileira de Cardiologia
Citation: LAROCCA, T. F. et al. Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy. Arquivos Brasileiros de Cardiologia, v. 100, n. 5, p. 460-468, 2013.
DOI: 10.5935/abc.20130058
ISSN: 1678-4170
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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