Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8568
Title: Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
Authors: Ferrazoli, Enéas Galdini
Blanco, Miriam Marcela
Bittencourt, Simone Ribeiro
Bachi, André Luis Lacerda
Bahia, Luciana Mendes Bahia
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Mello, Luiz Eugênio
Longo, Beatriz Monteiro
Affilliation: Federal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, Brasil
Federal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, Brasil
Federal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, Brasil
Federal University of São Paulo. UNIFESP. Departamento de Micro-Imuno-Parasitologia. São Paulo, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Federal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, Brasil
Federal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, Brasil
Abstract: BACKGROUND: Bone marrow is an accessible source of progenitor cells, which have been investigated as treatment for neurological diseases in a number of clinical trials. Here we evaluated the potential benefit of bone marrow cells in protecting against convulsive seizures induced by maximum electroconvulsive shock (MES), a widely used model for screening of anti-epileptic drugs. Behavioral and inflammatory responses were measured after MES induction in order to verify the effects promoted by transplantation of bone marrow cells. To assess the anticonvulsant effects of bone marrow cell transplantation, we measured the frequency and duration of tonic seizure, the mortality rate, the microglial expression and the blood levels of cytokine IL-1, IL-6, IL-10 and TNF-α after MES induction. We hypothesized that these behavioral and inflammatory responses to a strong stimulus such as a convulsive seizure could be modified by the transplantation of bone marrow cells. RESULTS: Bone marrow transplanted cells altered the convulsive threshold and showed anticonvulsant effect by protecting from tonic seizures. Bone marrow cells modified the microglial expression in the analyzed brain areas, increased the IL-10 and attenuate IL-6 levels. CONCLUSIONS: Bone marrow cells exert protective effects by blocking the course of electroconvulsive seizures. Additionally, electroconvulsive seizures induced acute inflammatory responses by altering the pattern of microglia expression, as well as in IL-6 and IL-10 levels. Our findings also indicated that the anticonvulsant effects of these cells can be tested with the MES model following the same paradigm used for drug testing in pharmacological screening. Studies on the inflammatory reaction in response to acute seizures in the presence of transplanted bone marrow cells might open a wide range of discussions on the mechanisms relevant to the pathophysiology of epilepsies.
Keywords: Bone marrow
Cell transplantation
Electroconvulsive shock
Tonic seizure
Microglia
Hippocampus
DeCS: Transplante de Medula Óssea/métodos
Encéfalo/citologia
Microglia/citologia
Convulsões/terapia
Animais
Citocinas/sangue
Eletrochoque/efeitos adversos
Imuno-Histoquímica
Camundongos
Camundongos Endogâmicos C57BL
Issue Date: 2013
Publisher: BioMed Central
Citation: FERRAZOLI, E. G. et al. Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice. BMC Neuroscience, v. 14, p. 97, 2013.
DOI: 10.1186/1471-2202-14-97
ISSN: 1471-2202
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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