| Author | Oliveira, Sheilla Andrade de | |
| Author | Souza, Bruno Solano de Freitas | |
| Author | Barreto, Elton Pereira Sá | |
| Author | Kaneto, Carla Martins | |
| Author | Almeida Neto, Hélio | |
| Author | Azevedo, Carine Machado | |
| Author | Guimarães, Elisalva Teixeira | |
| Author | Freitas, Luiz Antonio Rodrigues de | |
| Author | Santos, Ricardo Ribeiro dos | |
| Author | Soares, Milena Botelho Pereira | |
| Access date | 2014-10-09T19:21:46Z | |
| Available date | 2014-10-09T19:21:46Z | |
| Document date | 2012 | |
| Citation | OLIVEIRA, S. A. et al. Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis. Cytotherapy, v. 14, n. 3, p. 339-349, 2012. | pt_BR |
| ISSN | 1477-2566 | |
| URI | https://www.arca.fiocruz.br/handle/icict/8572 | |
| Language | eng | pt_BR |
| Publisher | Informa Healthcare | pt_BR |
| Rights | open access | pt_BR |
| Title | Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis. | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3109/14653249.2011.637668 | |
| Abstract | Background aims. Cirrhosis, end-stage liver disease, is caused by different mechanisms of injury, associated with persistent
infl ammation. Galectin-3 is an important regulator of fi brosis that links chronic infl ammation to fi brogenesis. We investigated
the role of bone marrow cell (BMC) transplantation in chronic infl ammation and hepatic fi brosis. Methods . Liver cirrhosis
was induced by administration of carbon tetrachloride and ethanol to wild-type C57BL/6 or bone marrow chimeric mice.
Bone marrow chimeras were generated by lethal irradiation and transplantation with BMC obtained from green fl uorescent
protein (GFP )donors. Wild-type cirrhotic mice were transplanted with BMC without irradiation. Livers from chimeras
and cirrhotic transplanted mice were obtained for evaluation of infl ammation, fi brosis and regulatory factors [galectin-3,
matrix metallopeptidase (MMP)-9, tissue inhibitor of metalloproteinase (TIMP)-1 and transforming growth factor (TGF)- β ].
Results . The development of cirrhosis was associated with increased expression of galectin-3 by F4/80 cells and intense
migration of BMC to the liver. Furthermore, when transplanted after the establishment of cirrhosis, BMC also migrated to
the liver and localized within the fi brous septa. Two months after BMC therapy, cirrhotic mice had a signifi cant reduction
in liver fi brosis and expression of type I collagen. We did not fi nd any difference in levels of TGF- β , TIMP-1 and MMP-9
between saline and BMC groups. However, the numbers of infl ammatory cells, phagocytes and galectin-3 cells were markedly
lower in the livers of cirrhotic mice treated with BMC. Conclusions . Our results demonstrate an important role for BMC
in the regulation of liver fi brosis and that transplantation of BMC can accelerate fi brosis regression through modulatory
mechanisms | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhaes. Recife, PE, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital São Rafael. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | pt_BR |
| Subject | Carbon tetrachloride | pt_BR |
| Subject | Galectin-3 | pt_BR |
| Subject | Hepatic cirrhosis | pt_BR |
| Subject | Mice | pt_BR |
| Subject | Stem cells | pt_BR |
| DeCS | Transplante de Medula Óssea/métodos | pt_BR |
| DeCS | Galectina 3/metabolismo | pt_BR |
| DeCS | Cirrose Hepática Experimental/terapia | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Células da Medula Óssea/citologia | pt_BR |
| DeCS | Células da Medula Óssea/metabolismo | pt_BR |
| DeCS | Tetracloreto de Carbono/administração & dosagem | pt_BR |
| DeCS | Movimento Celular | pt_BR |
| DeCS | Quimera | pt_BR |
| DeCS | Colágeno Tipo I/metabolismo | pt_BR |
| DeCS | Etanol/administração & dosagem | pt_BR |
| DeCS | Feminino | pt_BR |
| DeCS | Proteínas de Fluorescência Verde/metabolismo | pt_BR |
| DeCS | Inflamação | pt_BR |
| DeCS | Fígado/metabolismo | pt_BR |
| DeCS | Fígado/patologia | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Camundongos | pt_BR |
| DeCS | Metaloproteinase 9 da Matriz/metabolismo | pt_BR |
| DeCS | Fagócitos/metabolismo | pt_BR |
| DeCS | Inibidor Tecidual de Metaloproteinase-1/metabolismo | pt_BR |
| DeCS | Fator de Crescimento Transformador beta/metabolismo | pt_BR |
