Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8572
Title: Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
Authors: Oliveira, Sheilla Andrade de
Souza, Bruno Solano de Freitas
Barreto, Elton Pereira Sá
Kaneto, Carla Martins
Almeida Neto, Hélio
Azevedo, Carine Machado
Guimarães, Elisalva Teixeira
Freitas, Luiz Antonio Rodrigues de
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhaes. Recife, PE, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Abstract: Background aims. Cirrhosis, end-stage liver disease, is caused by different mechanisms of injury, associated with persistent infl ammation. Galectin-3 is an important regulator of fi brosis that links chronic infl ammation to fi brogenesis. We investigated the role of bone marrow cell (BMC) transplantation in chronic infl ammation and hepatic fi brosis. Methods . Liver cirrhosis was induced by administration of carbon tetrachloride and ethanol to wild-type C57BL/6 or bone marrow chimeric mice. Bone marrow chimeras were generated by lethal irradiation and transplantation with BMC obtained from green fl uorescent protein (GFP )donors. Wild-type cirrhotic mice were transplanted with BMC without irradiation. Livers from chimeras and cirrhotic transplanted mice were obtained for evaluation of infl ammation, fi brosis and regulatory factors [galectin-3, matrix metallopeptidase (MMP)-9, tissue inhibitor of metalloproteinase (TIMP)-1 and transforming growth factor (TGF)- β ]. Results . The development of cirrhosis was associated with increased expression of galectin-3 by F4/80 cells and intense migration of BMC to the liver. Furthermore, when transplanted after the establishment of cirrhosis, BMC also migrated to the liver and localized within the fi brous septa. Two months after BMC therapy, cirrhotic mice had a signifi cant reduction in liver fi brosis and expression of type I collagen. We did not fi nd any difference in levels of TGF- β , TIMP-1 and MMP-9 between saline and BMC groups. However, the numbers of infl ammatory cells, phagocytes and galectin-3 cells were markedly lower in the livers of cirrhotic mice treated with BMC. Conclusions . Our results demonstrate an important role for BMC in the regulation of liver fi brosis and that transplantation of BMC can accelerate fi brosis regression through modulatory mechanisms
Keywords: Carbon tetrachloride
Galectin-3
Hepatic cirrhosis
Mice
Stem cells
DeCS: Transplante de Medula Óssea/métodos
Galectina 3/metabolismo
Cirrose Hepática Experimental/terapia
Animais
Células da Medula Óssea/citologia
Células da Medula Óssea/metabolismo
Tetracloreto de Carbono/administração & dosagem
Movimento Celular
Quimera
Colágeno Tipo I/metabolismo
Etanol/administração & dosagem
Feminino
Proteínas de Fluorescência Verde/metabolismo
Inflamação
Fígado/metabolismo
Fígado/patologia
Masculino
Camundongos
Metaloproteinase 9 da Matriz/metabolismo
Fagócitos/metabolismo
Inibidor Tecidual de Metaloproteinase-1/metabolismo
Fator de Crescimento Transformador beta/metabolismo
Issue Date: 2012
Publisher: Informa Healthcare
Citation: OLIVEIRA, S. A. et al. Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis. Cytotherapy, v. 14, n. 3, p. 339-349, 2012.
DOI: 10.3109/14653249.2011.637668
ISSN: 1477-2566
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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