Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8683
Title: Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
Authors: Franco, Priscila Silva
Gomes, Angelica de Oliveira
Barbosa, Bellisa de Freitas
Angeloni, Mariana Bodini
Silva, Neide Maria
Carvalho, Andréa Teixeira
Martins Filho, Olindo Assis
Silva, Daise Aparecida Oliveira
Mineo, José Roberto
Affilliation: Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brazil
Abstract: Toxoplasma gondii is an important pathogen which may cause fetal infection if primary infection. Our previous studies have used human choriocarcinoma trophoblastic cells (BeWo cell line) as experimental model of T. gondii infection involving placental microenvironment. This study aimed to examine the effects of azithromycin and spiramycin against T. gondiiinfection in BeWo cells. Cells were treated with different concentrations of the macrolide antibiotics and analyzed first for cell viability using thiazolyl blue tetrazole (MTT) assay. As cell viability was significantly decreased with drug concentrations higher than 400 μg/mL, the concentration range used in further experiments was from 50 to 400 μg/mL. The number of infected cells and intracellular replication of T. gondii decreased after treatment with each drug. The infection induced up-regulation of the macrophage migration inhibitory factor (MIF), which was also enhanced in infected cells after treatment with azithromycin, but not with spiramycin. Analysis of the cytokine profile showed increase TNF-α, IL-10 and IL-4 production, but decreased IFN-γ levels, were detected in infected cells and treated with each drug. In conclusion, treatment of human trophoblastic BeWo cells with with azithromycin or spiramycin is able to control the infection and replication of T. gondii. In addition, treatment with these macrolides, especially with azityromycin induces an anti-inflammatory response and high MIF production, which can be important for the establishment and maintenance of a viable pregnancy during T. gondii infection.
Keywords: Azithromycin
Spiramycin
BeWo cells
Cytokines
Toxoplasma gondii
Issue Date: 2011
Publisher: Elsevier Ltd.
Citation: FRANCO, Priscila Silva et at. Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection. Placenta, 32(11): 838-844, 2011.
ISSN: 0143-4004
10.1016/j.placenta.2011.08.012
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos



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