Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8743
Title: CD4(+)T cells defined by their Vb T cell receptor expression are associated with immunoregulatory profiles and lesion size in human leishmaniasis
Authors: Lima, Tatjana Keesen de Souza
Antonelli, Lis Ribeiro do Valle
Barbosa, Daniela Rodrigues de Faria
Guimarães, Luiz Henrique Santos
Bacellar, Maria Olívia Amado Ramos
Carvalho Filho, Edgar Marcelino de
Dutra, Walderez Ornelas
Gollob, Kenneth John
Affilliation: Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonnte, MG, Brasil/Santa Casa Hospital. Instituto para Educação e Pesquisa. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brasil
Universidade Federal da Bahia. Hospital Edgard Santos. Immunology Service. Salvador, Brazil/ Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais
Universidade Federal da Bahia. Hospital Edgard Santos. Immunology Service. Salvador, Brazil/ Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais
Universidade Federal da Bahia. Hospital Edgard Santos. Immunology Service. Salvador, Brazil/ Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais
Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brasil/Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais
Santa Casa Hospital. Instituto para Educação e Pesquisa. Belo Horizonte, MG, Brasil/ Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais/SRI International. Biosciences Division. Center for Infections Disease Research. Menlo Park, CA, USA
Abstract: Leishmaniasis is caused by infection with the protozoan parasite, Leishmania, that parasitizes human cells, and the cellular immune response is essential for controlling infection. In order to measure the host T cell response to Leishmania infection, we have measured the expansion, activation state and functional potential of specific T cells as identified by their T cell receptor Vb region expression. In a group of cutaneous leishmaniasis (CL) patients, we evaluated these characteristics in nine different T cell subpopulations as identified by their Vb region expression, before and after specific Leishmania antigen stimulation. Our results show: (1) an increase in CD4+ T cells expressing Vb 5•2 and Vb 24 in CL compared to controls; (2) a Leishmania antigeninduced increase in CD4+ T cells expressing Vb 5•2, 11, 12 and 17; (3) a profile of previous activation of CD4+Vb 5•2-, 11- and 24-positive T cells,with higher expression of CD45RO, HLA-DR, interferon-g, tumour necrosis factor-a and interleukin-10 compared to other Vb-expressing subpopulations; (4) a positive correlation between higher frequencies of CD4+Vb5•2+ T cells and larger lesions; and (5) biased homing of CD4+ T cells expressing Vb 5•2 to the lesion site. Given that CL disease involves a level of pathology (ulcerated lesions) and is often followed by long-lived protection and cure, the identification of specific subpopulations active in this form of disease could allow for the discovery of immunodominant Leishmania antigens important for triggering efficient host responses against the parasite, or identify cell populations most involved in pathology.
Keywords: CD4 T cells
cytokines
human leishmaniasis
T cell receptor
tegumentary lesions
Issue Date: 2011
Publisher: 1365-2249
Citation: LIMA, Tatjana Keesen de Souza et al. CD4(+)T cells defined by their Vb T cell receptor expression are associated with immunoregulatory profiles and lesion size in human leishmaniasis. Clin Exp Immunol., 165(3): 338-351, 2011.
DOI: 10.1111/j.1365-2249.2011.04430.x
ISSN: M. Peakman
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos




FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.