Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8922
Title: Activation of sonic hedgehog signaling in oral squamous cell carcinomas: a preliminary study.
Authors: Buim, Marcilei Eliza Cavichiolli
Gurgel, Clarissa Araújo Silva
Ramos, Eduardo Antônio Gonçalves
Lourenço, Silvia Vanessa
Soares, Fernando Augusto
Affilliation: Hospital A.C. Camargo. Department of Anatomical Pathology. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
University of São Paulo. Dental School. Department of General Pathology. São Paulo, SP, Brasil
Hospital A.C. Camargo. Department of Anatomical Pathology. São Paulo, SP, Brasil / University of São Paulo. Dental School. Department of General Pathology. São Paulo, SP, Brasil
Abstract: Sonic hedgehog signaling is important for human development, and aberrant regulation of this pathway can result in the development of tumors. The aim of this study was to examine the expression of sonic hedgehog signaling molecules in oral squamous cell carcinoma. By quantitative real-time polymerase chain reaction, the expression of SHH, SMO, PTCH-1, and GLI-1 was analyzed in 30 oral squamous cell carcinoma cases and 8 samples of nonneoplastic oral mucosa and associated to clinical pathologic features. The expression of β-catenin, cyclin D1, Wnt-1, and Egfr was evaluated by immunohistochemistry in 26 available cases of oral squamous cell carcinoma. Normal oral mucosa from healthy individuals was negative for all genes that were evaluated. SHH, PTCH-1, SMO, and GLI-1 were not expressed in nonneoplastic oral mucosa, and low levels of GLI-1 were observed in nonneoplastic oral mucosa that was adjacent to the tumor. All oral squamous cell carcinoma cases expressed high levels of PTCH-1, SMO, and GLI-1 and were devoid of SHH. The expression of SMO was associated with clinical stage (P = .022) and a borderline association in cervical lymph node metastasis (P = .053). PTCH-1 expression showed a strong correlation with SMO (rs = 0.64; P b .001) and GL-1 (rs = 0.70; P b .001); SMO and GLI-1 also correlated with each other (rs, 0.55; P b .001). All proteins evaluated were expressed as cyclin D1 (92% of samples), β-catenin (73%), Egfr (46%), or Wnt- 1 (32%). Our data demonstrate that sonic hedgehog signaling is activated in oral squamous cell carcinoma and suggest that this pathway mediates its tumorigenesis.
Keywords: Sonic hedgehog
Oral squamous cell carcinoma
Gene expression
Immunohistochemistry
DeCS: Carcinoma de Células Escamosas/metabolismo
Proteínas Hedgehog/fisiologia
Neoplasias Bucais/metabolismo
Adulto
Idoso
Idoso de 80 Anos ou mais
Carcinoma de Células Escamosas/patologia
Perfilação da Expressão Gênica
Feminino
Humanos
Imuno-Histoquímica
Neoplasias Bucais/patologia
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Transdução de Sinal
Issue Date: 2011
Publisher: Elsevier Inc
Citation: BUIM, M. E. C. et al. Activation of sonic hedgehog signaling in oral squamous cell carcinomas: a preliminary study. Human Pathology, v. 42, n. 10, p. 1484-1490, 2011.
DOI: 10.1016/j.humpath.2010.12.015
ISSN: 1532-8392
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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