Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8931
Title: Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
Authors: Martins, Gustavo Ferreira
Guedes, Bruno Augusto Maciel
Silva, Luciana Maria
Serrão, José Eduardo
Dias, Consuelo Latorre Fortes
Ortigão, José Marcelo Ramalho
Pimenta, Paulo Filemon Paolucci
Affilliation: Universidade Federal de Viçosa. Departamento de Biologia Geral. Laboratório de Biologia Celular. Viçosa, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil/Universidades Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Laboratório de Biologia Celular. Belo Horizonte, MG, Brazil
Universidade Federal de Viçosa. Departamento de Biologia Geral. Laboratório de Biologia Celular. Viçosa, MG, Brazil
Fundação Ezequiel Dias. Laboratório de Biologia Celular. Belo Horizonte, MG, Brazil
Kansas State University. Department of Entomology. Manhattan, KS, USA
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil
Abstract: Oenocytes are ectodermic cells that participate in a number of critical physiological roles such as detoxification and lipid storage and metabolism in insects. In light of the lack of information on oenocytes from Aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain Ae. aegypti pupa oenocytes in primary culture. Ae. aegypti oenocytes were cultured as clustered and as isolated ovoid cells with a smooth surface. Our results demonstrate that these cells remain viable in cell culture for at least two months. We also investigated their morphology in vivo and in vitro using light, confocal, scanning and transmission electron microscopes. This work is the first successful attempt in isolating and maintaining Ae. aegypti oenocytes in culture, and a significant step towards understanding the role of this cell type in this important disease vector. The purification and the development of primary cultures of insect oenocytes will allow future studies of their metabolism in producing and secreting compounds.
Keywords: Aedes aegypti
Oenocyte
Primary culture
Morphology
Mosquito
Issue Date: 2011
Publisher: Oliver & Boyd.
Citation: MARTINS, Gustavo Ferreira et al. Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae. Tissue & Cell, v. 43, n. 2, p. 83-90, 2011.
DOI: 10.1016/j.tice.2010.12.003
ISSN: 0040-8166
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos




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