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AutorCarvalho, Isabel Maria Vicente Guedes de
AutorQueiroz, Artur Trancoso Lopo de
AutorMoraes, Rosiane Brito de
AutorGil, Helio Benites
AutorAlves, Rafael
AutorViviani, Andréa de Barros Pinto
AutorBecnel, James John
AutorCoutinho, Carlos José Pereira da Cunha de Araujo
Fecha de acceso2014-12-10T19:06:45Z
Fecha de disponibilización2014-12-10T19:06:45Z
Fecha de publicación2014
ReferenciaCARVALHO, I. M. et al. Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae). Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 5, p. 624-631, 2014.pt_BR
ISSN0037-8682
URIhttps://www.arca.fiocruz.br/handle/icict/9143
Idiomaengpt_BR
EditorSociedade Brasileira de Medicina Tropicalpt_BR
Derechos de autoropen accesspt_BR
TítuloDescription of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)pt_BR
Tipo del documentoArticlept_BR
DOIdx.doi.org/10.1590/0037-8682-0165-2014
Resumen en InglésIntroduction: Microsporidia constitute the most common black fl y pathogens, although the species’ diversity, seasonal occurrence and transmission mechanisms remain poorly understood. Infections by this agent are often chronic and non-lethal, but they can cause reduced fecundity and decreased longevity. The objective of this study was to identify microsporidia infecting Simulium (Chirostilbia) pertinax (Kollar, 1832) larvae from Caraguatatuba, State of São Paulo, Brazil, by molecular and morphological characterization. Methods: Larvae were collected at a single point in a stream in a rural area of the city and were kept under artifi cial aeration until analysis. Polydispyrenia spp. infection was characterized by the presence of at least 32 mononuclear spores measuring 6.9 ± 1.0 x 5.0 ± 0.7μm in persistent sporophorous vesicles. Similarly, Amblyospora spp. were characterized by the presence of eight uninucleate spores measuring 4.5 x 3.5μm in sporophorous vesicles. Results: The molecular analysis confi rmed the presence of microsporidian DNA in the 8 samples (prevalence of 0.51%). Six samples (Brazilian larvae) were related to Polydispyrenia simulii and Caudospora palustris reference sequences but in separate clusters. One sample was clustered with Amblyospora spp. Edhazardia aedis was the positive control taxon. Conclusions: Samples identifi ed as Polydispyrenia spp. and Amblyospora spp. were grouped with P. simulii and Amblyospora spp., respectively, corroborating previous results. However, the 16S gene tree showed a considerable distance between the black fl y-infecting Amblyospora spp. and the mosquito-infecting spp. This distance suggests that these two groups are not congeneric. Additional genomic region evaluation is necessary to obtain a coherent phylogeny for this group.pt_BR
AfiliaciónInstituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, Brasilpt_BR
AfiliaciónSuperintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, Brasilpt_BR
AfiliaciónUniversidade Federal de São Paulo. Disciplina de Infectologia. São Paulo, SP, Brasilpt_BR
AfiliaciónUniversidade Federal de São Paulo. Departamento de Medicina. Disciplina de Gastroenterologia. São Paulo, SP, Brasilpt_BR
AfiliaciónSuperintendência de Controle de Endemias. Laboratório de Simulídeos, Caraguatatuba, SP, Brasilpt_BR
AfiliaciónUnited States Department of Agriculture Mosquito. Florida, USA / Fly Research Unit. Gainesville, Florida, USApt_BR
AfiliaciónSuperintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, Brasilpt_BR
Palavras clave en InglêsMicrosporidaept_BR
Palavras clave en InglêsAmblyospora spppt_BR
Palavras clave en InglêsPolydispyrenia spppt_BR
Palavras clave en InglêsPhylogenetic analysispt_BR


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