Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9143
Title: Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
Authors: Carvalho, Isabel Maria Vicente Guedes de
Queiroz, Artur Trancoso Lopo de
Moraes, Rosiane Brito de
Gil, Helio Benites
Alves, Rafael
Viviani, Andréa de Barros Pinto
Becnel, James John
Coutinho, Carlos José Pereira da Cunha de Araujo
Affilliation: Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, Brasil
Superintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Disciplina de Infectologia. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Gastroenterologia. São Paulo, SP, Brasil
Superintendência de Controle de Endemias. Laboratório de Simulídeos, Caraguatatuba, SP, Brasil
United States Department of Agriculture Mosquito. Florida, USA / Fly Research Unit. Gainesville, Florida, USA
Superintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, Brasil
Abstract: Introduction: Microsporidia constitute the most common black fl y pathogens, although the species’ diversity, seasonal occurrence and transmission mechanisms remain poorly understood. Infections by this agent are often chronic and non-lethal, but they can cause reduced fecundity and decreased longevity. The objective of this study was to identify microsporidia infecting Simulium (Chirostilbia) pertinax (Kollar, 1832) larvae from Caraguatatuba, State of São Paulo, Brazil, by molecular and morphological characterization. Methods: Larvae were collected at a single point in a stream in a rural area of the city and were kept under artifi cial aeration until analysis. Polydispyrenia spp. infection was characterized by the presence of at least 32 mononuclear spores measuring 6.9 ± 1.0 x 5.0 ± 0.7μm in persistent sporophorous vesicles. Similarly, Amblyospora spp. were characterized by the presence of eight uninucleate spores measuring 4.5 x 3.5μm in sporophorous vesicles. Results: The molecular analysis confi rmed the presence of microsporidian DNA in the 8 samples (prevalence of 0.51%). Six samples (Brazilian larvae) were related to Polydispyrenia simulii and Caudospora palustris reference sequences but in separate clusters. One sample was clustered with Amblyospora spp. Edhazardia aedis was the positive control taxon. Conclusions: Samples identifi ed as Polydispyrenia spp. and Amblyospora spp. were grouped with P. simulii and Amblyospora spp., respectively, corroborating previous results. However, the 16S gene tree showed a considerable distance between the black fl y-infecting Amblyospora spp. and the mosquito-infecting spp. This distance suggests that these two groups are not congeneric. Additional genomic region evaluation is necessary to obtain a coherent phylogeny for this group.
Keywords: Microsporidae
Amblyospora spp
Polydispyrenia spp
Phylogenetic analysis
Issue Date: 2014
Publisher: Sociedade Brasileira de Medicina Tropical
Citation: CARVALHO, I. M. et al. Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae). Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 5, p. 624-631, 2014.
DOI: dx.doi.org/10.1590/0037-8682-0165-2014
ISSN: 0037-8682
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Carvalho IMVG Description....pdf2.33 MBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.