Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9170
Title: Performance of rapid hepatitis C virus antibody assays among high- and low-risk populations
Authors: Scalioni, Leticia de Paula
Cruz, Helena Medina
Paula, Vanessa Salete de
Miguel, Juliana Custódio
Marques, Vanessa Alves
Nogueira, Cristiane Alves Villela
Milagres, Flavio Augusto Pádua
Cruz, Marcelo Santos
Bastos, Francisco Inácio Pinkusfeld Monteiro
Andrade, Tarcisio Matos
Castro, Ana Rita Coimbra Motta
Ximenez, Lia Laura Lewis
Lampe, Elisabeth
Villar, Livia Melo
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga. Divisão de Hepatologia, Rio de Janeiro, RJ, Brasil.
Universidade Federal do Tocantins. Faculdade de Medicina. Palmas, TO, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Psiquiatria. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Comunicação e Informação Cientifica e Tecnológica em Saúde. Rio de Janeiro, RJ, Brasil.
Universidade Federal da Bahia. Departamento de Saúde da Família. Salvador, BA, Brasil.
Universidade Federal do Mato Grosso do Sul. Departamento de Farmácia Bioquímica. Campo Grande, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Abstract: Background: Rapid tests for the detection of antibodies to hepatitis C virus (anti-HCV) can facilitate accessto diagnosis.Objectives: This study aimed to evaluate the performance of rapid tests for anti-HCV detection in thesera, whole blood, and oral fluid samples from individuals with different endemicity profiles and riskbehaviors.Study design: Three groups donated biological samples that were tested using three anti-HCV rapid tests(WAMA, Bioeasy and OraSure): (I) suspected cases of hepatitis C, (II) individuals who were living inremote areas in Brazil and (III) crack users and beauty professionals. Reproducibility, repeatability andcross-reactivity to other infectious agents (dengue, HIV, malaria, and syphilis) were also evaluated.Results: In group I, specificities varied from 93.75% to 100% and sensitivities varied from 76.03% to 93.84%according to the EIA results. When anti-HCV/HCV RNA-reactive sera samples were considered true-positive HCV cases, the sensitivities and specificities varied from 86.3% to 99.09% and 93.75% to 100%,respectively. In group II, the OraSure rapid test presented the best performance. In group III, the Bioeasyassay performed best using saliva and whole blood and the OraSure assay performed best using oral fluidsamples. The reproducibility and repeatability of the WAMA and Bioeasy tests were excellent. The levelof concordance between the HCV EIAs and the rapid tests using samples that were reactive for otherinfectious agents varied from 82.35% to 100% for the WAMA assay and 94.11% to 100% for the Bioeasyassay.Conclusion: All of the rapid tests could be used to identify active HCV infection among individuals withdifferent endemicity profiles and risk behaviors.
keywords: Testes Rápidos
DeCS: Hepatite C /diagnóstico
Testes Sorológicos
Doenças Endêmicas
Prevalência
Anticorpos Anti-Hepatite C
Issue Date: 2014
Publisher: Elsevier
Citation: SCALIONI, L. de P. Performance of rapid hepatitis C virus antibody assays among high- and low-risk populations. Journal of Clinical Virology. v.60, n.3, p. 200-205, 2014
DOI: http://dx.doi.org/10.1016/j.jcv.2014.04.001
ISSN: 1386-6532
Copyright: restricted access
Appears in Collections:ICICT - Artigos de Periódicos
IOC - Artigos de Periódicos




FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.