Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9289
Title: Multi-centric prospective evaluation of rk39 rapid test and direct agglutination test for the diagnosis of visceral leishmaniasis in Brazil
Authors: Assis, Tália Santana Machado de
Braga, Alexandre Sérgio da Costa
Pedras, Mariana Junqueira
Oliveira, Edward José de
Barral, Aldina Maria Prado
Siqueira, Isadora Cristina de
Costa, Carlos Henrique Nery
Costa, Dorcas Lamounier
Holanda, Thiago Ayres
Soares, Vítor Yamashiro Rocha
Silva, Mauro Roberto Biá da
Caldas, Arlene de Jesus Mendes
Romero, Gustavo Adolfo Sierra
Rabello, Ana Lúcia Teles
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital da Criança. Obras Sociais de Irmã Dulce. Salvador, BA, Brasil
Universidade Federal do Piauí. Teresina, PI, Brasil
Universidade Federal do Piauí. Teresina, PI, Brasil
Universidade Federal do Piauí. Teresina, PI, Brasil
Universidade Federal do Piauí. Teresina, PI, Brasil
Universidade Federal do Piauí. Teresina, PI, Brasil
Universidade Federal do Maranhão. São Luís, MA, Brasil
Universidade de Brasília. Brasília, DF, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil
Abstract: The diagnosis of visceral leishmaniasis (VL) is still a major problem in Brazil and several other countries where the disease is endemic. The use of an easy-to-use and interpret, sensitive, and specific method that requires no complex infrastructure or specialized professionals, such as direct agglutination test (DAT) and the rK39-based rapid immunochromatographic test may enhance the diagnosis of disease. This study evaluated the performance of a rapid test (DiaMed- IT-LEISH®) and the DAT for the diagnosis of VL in 213 parasitologically confirmed cases and 119 controls with clinical suspicion of VL and confirmation of another etiology. The sensitivities and specificities of the rapid test were 93% and 97%, respectively and those of the DAT were 90% and 96%, respectively. The positive predictive values of the rapid test and the DAT were 98% and 97%, respectively and the negative predictive values were 89% and 84%, respectively. The Kappa index showed agreement between both methods classified as substantial (0.77). This study showed that the DAT and the rapid test can be used to diagnose VL in Brazil, following a pilot study for implementation of the rapid test in the health services
Keywords: Direct agglutination test
Rapid test rK39
Visceral leishmaniasis
Sensitivity
Specificity and diagnostic accuracy
DeCS: Testes de Aglutinação/normas
Leishmaniose Visceral/diagnóstico
Kit de Reagentes para Diagnóstico
Animais
Brasil/epidemiologia
Feminino
Humanos
Masculino
Prevalência
Leishmaniose Visceral/epidemiologia
Estudos Prospectivos
Padrões de Referência
Sensibilidade e Especificidade
Issue Date: 2011
Publisher: Oxford University Press
Citation: ASSIS, T. S. M. et al. Multi-centric prospective evaluation of rk39 rapid test and direct agglutination test for the diagnosis of visceral leishmaniasis in Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 105, n. 2, p. 81-85, 2011.
DOI: 10.1016/j.trstmh.2010.09.004
ISSN: 1878-3503
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Assis T S M Multi-centric prospective....pdf134.92 kBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.