| Autor | Lima, Alessandra Estrela da Silva | |
| Autor | Araújo, Márcio Sobreira Silva | |
| Autor | Costa Neto, João Moreira | |
| Autor | Carvalho, Andréa Teixeira | |
| Autor | Melo, Stella Maria Barrouin | |
| Autor | Cardoso, Sergio Vitorino | |
| Autor | Martins Filho, Olindo Assis | |
| Autor | Serakides, Rogéria | |
| Autor | Cassali, Geovanni Dantas | |
| Data de acesso | 2015-01-14T11:16:13Z | |
| Data de disponibilização | 2015-01-14T11:16:13Z | |
| Data do publicação | 2010 | |
| Citação | LIMA, Alessandra Estrela da Silva et al. Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates. BMC Câncer, 10(1): 256, 2010. | pt_BR |
| ISSN | 1471-2407 | |
| URI | https://www.arca.fiocruz.br/handle/icict/9379 | |
| Idioma | eng | pt_BR |
| Editor | BioMed Central | pt_BR |
| Direito Autoral | open access | pt_BR |
| Título | Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates | pt_BR |
| Tipo do documento | Article | pt_BR |
| DOI | 10.1186/1471-2407-10-256 | |
| Resumo em Inglês | Background: The immune system plays an important role in the multifactorial biologic system during the development of neoplasias. However, the involvement of the inflammatory response in the promotion/control of malignant cells is still controversial, and the cell subsets and the mechanisms involved are poorly investigated. The goal of this study was to characterize the clinical-pathological status and the immunophenotyping profile of tumor infiltrating lymphocytes and their association with the animal survival rates in canine mammary carcinomas. Methods: Fifty-one animals with mammary carcinomas, classified as carcinomas in mixed tumors-MC-BMT = 31 and carcinomas-MC = 20 were submitted to systematic clinical-pathological analysis (tumor size; presence of lymph node and pulmonary metastasis; clinical stage; histological grade; inflammatory distribution and intensity as well as the lymphocytic infiltrate intensity) and survival rates. Twenty-four animals (MC-BMT = 16 and MC = 8) were elected to the immunophenotypic study performed by flow cytometry. Results: Data analysis demonstrated that clinical stage II-IV and histological grade was I more frequent in MC-BMT as compared to MC. Univariate analysis demonstrated that the intensity of inflammation (moderate/intense) and the proportion of CD4+ (≥ 66.7%) or CD8+ T-cells (<33.3%) were not associated with worse survival rate. Multivariate analysis demonstrated that only lymphocytic infiltrate intensity ≥ 600 (P = 0.02) remained as independent prognostic factor. Despite the clinical manifestation, the lymphocytes represented the predominant cell type in the tumor infiltrate. The percentage of T-cells was higher in animals with MC-BMT without metastasis, while the percentage of Blymphocytes was greater in animals with metastasized MC-BMT (P < 0.05). The relative percentage of CD4+ T-cells was significantly greater in metastasized tumors (both MC-BMT and MC), (P < 0.05) while the proportion of CD8+ T-cells was higher in MC-BMT without metastasis. Consequently, the CD4+/CD8+ ratio was significantly increased in both groups with metastasis. Regardless of the tumor type, the animals with high proportions of CD4+ and low CD8+ T-cells had decreased survival rates. Conclusion: The intensity of lymphocytic infiltrate and probably the relative abundance of the CD4+ and CD8+ Tlymphocytes may represent important survival prognostic biomarkers for canine mammary carcinomas. | pt_BR |
| Afiliação | Universidade Federal da Bahia. Escola de Medicina Veterinária. Departamento de Patologia e Clínicas. Salvador, BA, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Laboratório de Patologia Comparada. Belo Horizonte, MG, Brasil | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil | pt_BR |
| Afiliação | Universidade Federal da Bahia. Escola de Medicina Veterinária. Departamento de Patologia e Clínicas. Salvador, BA, Brasil | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil | pt_BR |
| Afiliação | Universidade Federal da Bahia. Escola de Medicina Veterinária. Departamento de Patologia e Clínicas. Salvador, BA, Brasil | pt_BR |
| Afiliação | Universidade Federal de Uberlândia. Faculdade de Odontologia. Área de Patologia. Uberlândia, MG, Brasil | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clinicas e Cirurgia. Belo Horizonte, MG, Brasil | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Laboratório de Patologia Comparada. Belo Horizonte, MG, Brasil | pt_BR |
| Palavras-chave em inglês | Neoplasias | pt_BR |
| Palavras-chave em inglês | immunophenotyping | pt_BR |
