Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9379
Title: Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates
Authors: Lima, Alessandra Estrela da Silva
Araújo, Márcio Sobreira Silva
Costa Neto, João Moreira
Carvalho, Andréa Teixeira
Melo, Stella Maria Barrouin
Cardoso, Sergio Vitorino
Martins Filho, Olindo Assis
Serakides, Rogéria
Cassali, Geovanni Dantas
Affilliation: Universidade Federal da Bahia. Escola de Medicina Veterinária. Departamento de Patologia e Clínicas. Salvador, BA, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Laboratório de Patologia Comparada. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Universidade Federal da Bahia. Escola de Medicina Veterinária. Departamento de Patologia e Clínicas. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Universidade Federal da Bahia. Escola de Medicina Veterinária. Departamento de Patologia e Clínicas. Salvador, BA, Brasil
Universidade Federal de Uberlândia. Faculdade de Odontologia. Área de Patologia. Uberlândia, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clinicas e Cirurgia. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Laboratório de Patologia Comparada. Belo Horizonte, MG, Brasil
Abstract: Background: The immune system plays an important role in the multifactorial biologic system during the development of neoplasias. However, the involvement of the inflammatory response in the promotion/control of malignant cells is still controversial, and the cell subsets and the mechanisms involved are poorly investigated. The goal of this study was to characterize the clinical-pathological status and the immunophenotyping profile of tumor infiltrating lymphocytes and their association with the animal survival rates in canine mammary carcinomas. Methods: Fifty-one animals with mammary carcinomas, classified as carcinomas in mixed tumors-MC-BMT = 31 and carcinomas-MC = 20 were submitted to systematic clinical-pathological analysis (tumor size; presence of lymph node and pulmonary metastasis; clinical stage; histological grade; inflammatory distribution and intensity as well as the lymphocytic infiltrate intensity) and survival rates. Twenty-four animals (MC-BMT = 16 and MC = 8) were elected to the immunophenotypic study performed by flow cytometry. Results: Data analysis demonstrated that clinical stage II-IV and histological grade was I more frequent in MC-BMT as compared to MC. Univariate analysis demonstrated that the intensity of inflammation (moderate/intense) and the proportion of CD4+ (≥ 66.7%) or CD8+ T-cells (<33.3%) were not associated with worse survival rate. Multivariate analysis demonstrated that only lymphocytic infiltrate intensity ≥ 600 (P = 0.02) remained as independent prognostic factor. Despite the clinical manifestation, the lymphocytes represented the predominant cell type in the tumor infiltrate. The percentage of T-cells was higher in animals with MC-BMT without metastasis, while the percentage of Blymphocytes was greater in animals with metastasized MC-BMT (P < 0.05). The relative percentage of CD4+ T-cells was significantly greater in metastasized tumors (both MC-BMT and MC), (P < 0.05) while the proportion of CD8+ T-cells was higher in MC-BMT without metastasis. Consequently, the CD4+/CD8+ ratio was significantly increased in both groups with metastasis. Regardless of the tumor type, the animals with high proportions of CD4+ and low CD8+ T-cells had decreased survival rates. Conclusion: The intensity of lymphocytic infiltrate and probably the relative abundance of the CD4+ and CD8+ Tlymphocytes may represent important survival prognostic biomarkers for canine mammary carcinomas.
Keywords: Neoplasias
immunophenotyping
Issue Date: 2010
Publisher: BioMed Central
Citation: LIMA, Alessandra Estrela da Silva et al. Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates. BMC Câncer, 10(1): 256, 2010.
DOI: 10.1186/1471-2407-10-256
ISSN: 1471-2407
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos



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