Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9398
Title: Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
Authors: Santos, Ethel Bayer
Lima, Fábio Mitsuo
Ruiz, Jeronimo Conceição
Almeida, Igor Correa
Silveira, José Franco da
Affilliation: Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil/University of Texas at El Paso. Border Biomedical Research Center. Department of Biological Sciences. El Paso, TX, USA
Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil
University of Texas at El Paso. Border Biomedical Research Center. Department of Biological Sciences. El Paso, TX, USA
Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil
Abstract: A growing body of evidence in mammalian cells indicates that secreted vesicles can be used to mediate intercellular communication processes by transferring various bioactive molecules, including mRNAs and microRNAs. Based on these findings, we decided to analyze whether Trypanosoma cruzi-derived extracellular vesicles contain RNA molecules and performed a deep sequencing and genome-wide analysis of a size-fractioned cDNA library (16–40 nt) from extracellular vesicles secreted by noninfective epimastigote and infective metacyclic trypomastigote forms. Our data show that the small RNAs contained in these extracellular vesicles originate from multiple sources, including tRNAs. In addition, our results reveal that the variety and expression of small RNAs are different between parasite stages, suggesting diverse functions. Taken together, these observations call attention to the potential regulatory functions that these RNAs might play once transferred between parasites and/or to mammalian host cells.
Keywords: Trypanosoma cruzi
Extracellular vesicles
Small noncoding RNA
High-throughput data analysis
Comparative transcriptome
Issue Date: 2014
Publisher: Elsevier Ltda
Citation: SANTOS, Ethel Bayer et al. Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles. Molecular & Biochemical Parasitology; v. 193, n. 2. p. 71–74, 2014
DOI: 10.1016/j.molbiopara.2014.02.004
ISSN: 0166-6851
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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