Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9492
Title: Variation of cytokine patterns related to therapeutic response in diffuse cutaneous leishmaniasis.
Authors: Arruda, Maria da Gloria Bomfim
Nascimento, Cristiane Santos
Costa, Jackson Mauricio Lopes
Carvalho Filho, Edgar Marcelino
Barral Netto, Manoel
Barral, Aldina Maria Prado
Affilliation: Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal do Maranhão. Faculdade de Medicina. Departamento de Doenças Infecciosas e Parasitárias. São Luis, MA, Brasil.
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.
Abstract: Diffuse cutaneous leishmaniasis is a rare entity characterized by disseminated cutaneous nodules associated with specific anergy to leishmanial antigens. A low but not absent IFN-gamma expression by cells present in cutaneous lesions has been documented during the active phase of diffuse cutaneous leishmaniasis. In this study we confirm this observation, and extend it by showing a similar pattern in peripheral blood mononuclear cells and the variation of mRNA cytokine expression pattern during different stages of the disease. During active disease, patients did not express mRNA for IFN-gamma, while expressing mRNA for IL-2, IL-4, and IL-10. In contrast, an expression of IFN-gamma and low IL-10 was observed after treatment-induced transient healing of cutaneous lesions. In three patients we have been able to analyze a third PBMC sample obtained after clinical relapse, documenting in all of them decreased IFN-gamma expression with no expression of IL-10. Although there was an association between the appearance of IFN-gamma expression and clinical improvement, with marked expression of IFN-gamma mRNA and decreased expression of mRNA for IL-10 after treatment, this was not sufficient to prevent relapse in these patients. Therefore, it is possible that factors other than the cytokines characteristic of the Th1 and Th2 balance are implicated in the inability of diffuse cutaneous leishmaniasis patients to mount an anti-Leishmania immune response causing clinical improvement.
Keywords: Leishmaniasis
Cytokines
Therapy
Cutaneous leishmaniasis
DCL
Diffuse cutaneous leishmaniasis
DeCS: Interferon gama/biossíntese
Interleucinas/biossíntese
Leishmaniose Tegumentar Difusa/imunologia
Adolescente
Adulto
Antiprotozoários/uso terapêutico
Criança
Feminino
Humanos
Ensaio de Imunoadsorção Enzimática
Leishmaniose Tegumentar Difusa/quimioterapia
Masculino
Reação em Cadeia da Polimerase
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Recidiva
Issue Date: 1996
Publisher: Elsevier
Citation: ARRUDA, Maria da Gloria Bomfim et al. Variation of cytokine patterns related to therapeutic response in diffuse cutaneous leishmaniasis. Experimental Parasitology, v. 84, n. 2, p. 188-194, 1996.
ISSN: 0014-4894
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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