Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9584
Title: Induction of IL-12 Production in Human Peripheral Monocytes by Trypanosoma cruzi Is Mediated by Glycosylphosphatidylinositol-Anchored Mucin-Like Glycoproteins and Potentiated by IFN-𝛾 and CD40-CD40L Interactions.
Authors: Abel, Lúcia Cristina Jamli
Ferreira, Ludmila Rodrigues Pinto
Navarro, Isabela Cunha
Baron, Monique Andrade
Kalil, Jorge
Gazzinelli, Ricardo Tostes
Rizzo, Luiz Vicente
Cunha Neto, Edecio
Affilliation: Universidade de São Paulo. Escola de Medicina. Instituto do Coração. Laboratory of Immunology. São Paulo, SP, Brazil
Universidade de São Paulo. Escola de Medicina. Instituto do Coração. Laboratory of Immunology. São Paulo, SP, Brazil/Universidade de São Paulo. Escola de Medicina. Clinca de Imunologia e Alergia. Sao Paulo, SP, Brazil/Institutos Nacionais de Ciência e Tecnologia. Instituto de Investigação em Imunologia. Sao Paulo, SP, Brazil
Universidade de São Paulo. Escola de Medicina. Instituto do Coração. Laboratory of Immunology. São Paulo, SP, Brazil/Universidade de São Paulo. Escola de Medicina. Clinca de Imunologia e Alergia. Sao Paulo, SP, Brazil/Institutos Nacionais de Ciência e Tecnologia. Instituto de Investigação em Imunologia. Sao Paulo, SP, Brazil
Universidade de São Paulo. Escola de Medicina. Instituto do Coração. Laboratory of Immunology. São Paulo, SP, Brazil/Universidade de São Paulo. Escola de Medicina. Clinca de Imunologia e Alergia. Sao Paulo, SP, Brazil/Institutos Nacionais de Ciência e Tecnologia. Instituto de Investigação em Imunologia. Sao Paulo, SP, Brazil
Universidade de São Paulo. Escola de Medicina. Instituto do Coração. Laboratory of Immunology. São Paulo, SP, Brazil/Universidade de São Paulo. Escola de Medicina. Clinca de Imunologia e Alergia. Sao Paulo, SP, Brazil/Institutos Nacionais de Ciência e Tecnologia. Instituto de Investigação em Imunologia. Sao Paulo, SP, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas.Departamento de Bioquimica e Imunologia. Laboratorio de Imunopatologia. Belo Horizonte, MG, Brazil/University of Massachusetts Medical School. Department of Medicine. Division of Infectious Diseases and Immunology. Worcester, MA, USA
Hospital Israelita Albert Einstein. Sao Paulo, SP, Brazil
Universidade de São Paulo. Escola de Medicina. Instituto do Coração. Laboratory of Immunology. São Paulo, SP, Brazil/Universidade de São Paulo. Escola de Medicina. Clinca de Imunologia e Alergia. Sao Paulo, SP, Brazil/Institutos Nacionais de Ciência e Tecnologia. Instituto de Investigação em Imunologia. Sao Paulo, SP, Brazil
Abstract: Chagas disease, caused by the protozoan parasite Trypanosoma cruzi (T. cruzi), is characterized by immunopathology driven by IFN-γ secreting Th1-like T cells. T. cruzi has a thick coat of mucin-like glycoproteins covering its surface, which plays an important role in parasite invasion and host immunomodulation. It has been extensively described that T. cruzi or its products-like GPI anchors isolated from GPI-anchored mucins from the trypomastigote life cycle stage (tGPI-mucins)-are potent inducers of proinflammatory responses (i.e., cytokines and NO production) by IFN-γ primed murine macrophages. However, little is known about whether T. cruzi or GPI-mucins exert a similar action in human cells. We therefore decided to further investigate the in vitro cytokine production profile from human mononuclear cells from uninfected donors exposed to T. cruzi as well as tGPI-mucins. We observed that both living T. cruzi trypomastigotes and tGPI-mucins are potent inducers of IL-12 by human peripheral blood monocytes and this effect depends on CD40-CD40L interaction and IFN-γ. Our findings suggest that the polarized T1-type cytokine profile seen in T. cruzi infected patients might be a long-term effect of IL-12 production induced by lifelong exposure to T. cruzi tGPI-mucins.
Keywords: Chagas disease
Trypanosoma cruzi
Issue Date: 2014
Publisher: Hindawi Publishing Corporation
Citation: ABEL, Lúcia Cristina Jamli et al. Induction of IL-12 Production in Human Peripheral Monocytes by Trypanosoma cruzi Is Mediated by Glycosylphosphatidylinositol-Anchored Mucin-Like Glycoproteins and Potentiated by IFN-𝛾 and CD40-CD40L Interactions. Mediators of Inflammation, V. 2014, Article ID 345659, 7 p., 2014
DOI: 10.1155/2014/345659
ISSN: 0962-9351
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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