Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9586
Title: Subdoses of 17DD yellow fever vaccine elicit equivalent virological/immunological kinetics timeline
Authors: Azevedo, Ana Carolina Campi
Estevam, Paula de Almeida
Reis, Jordana Grazziela Alves Coelho dos
Magalhães, Vanessa Peruhype
Rezende, Gabriela Villela
Quaresma, Patrícia Flávia
Maia, Maria de Lourdes Sousa
Farias, Roberto Henrique Guedes
Camacho, Luiz Antonio Bastos
Galler, Ricardo
Yamamura, Anna Maya Yoshida
Almeida, Luiz Fernando Carvalho
Lima, Sheila Maria Barbosa
Nogueira, Rita Maria Ribeiro
Sá, Gloria Regina Silva
Hokama, Darcy Akemi
Carvalho, Ricardo de
Freire, Ricardo Aguiar Villanova
Pereira Filho, Edson
Leal, Maria da Luz Fernandes
Homma, Akira
Carvalho, Andréa Teixeira
Martins, Reinaldo de Menezes
Martins Filho, Olindo Assis
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Minas Gerais, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Minas Gerais, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Minas Gerais, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Minas Gerais, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Minas Gerais, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Minas Gerais, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro,RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Instituto de Biologia do Exército. Rio de Janeiro, RJ, Brasil.
Instituto de Biologia do Exército. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Minas Gerais, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Minas Gerais, Brasil.
Abstract: Background: The live attenuated 17DD Yellow Fever vaccine is one of the most successful prophylactic interventions for controlling disease expansion ever designed and utilized in larger scale. However, increase on worldwide vaccine demands and manufacturing restrictions urge for more detailed dose sparing studies. The establishment of complementary biomarkers in addition to PRNT and Viremia could support a secure decision-making regarding the use of 17DD YF vaccine subdoses. The present work aimed at comparing the serum chemokine and cytokine kinetics triggered by five subdoses of 17DD YF Vaccine. Methods: Neutralizing antibody titers, viremia, cytokines and chemokines were tested on blood samples obtained from eligible primary vaccinees. Results and discussion: The results demonstrated that a fifty-fold lower dose of 17DD-YF vaccine (587 IU) is able to trigger similar immunogenicity, as evidenced by significant titers of anti-YF PRNT. However, only subdoses as low as 3,013 IU elicit viremia kinetics with an early peak at five days after primary vaccination equivalent to the current dose (27,476 IU), while other subdoses show a distinct, lower in magnitude and later peak at day 6 post-vaccination. Although the subdose of 587 IU is able to trigger equivalent kinetics of IL-8/CXCL-8 and MCP-1/CCL-2, only the subdose of 3,013 IU is able to trigger similar kinetics of MIG/CXCL-9, pro-inflammatory (TNF, IFN-γ and IL-2) and modulatory cytokines (IL-5 and IL-10). Conclusions: The analysis of serum biomarkers IFN-γ and IL-10, in association to PRNT and viremia, support the recommendation of use of a ten-fold lower subdose (3,013 IU) of 17DD-YF vaccine.
Keywords: Yellow fever
Vaccine
Dose–response
Viremia
Cytokines
Ckemokines
Issue Date: 2014
Publisher: BioMed Central
Citation: AZEVEDO, Ana Carolina Campi et al. Subdoses of 17DD yellow fever vaccine elicit equivalent virological/immunological kinetics timeline. BMC Infectious Diseases, v. 14, p. 1-12, 2014.
DOI: 10.1186/1471-2334-14-391
ISSN: 471-2334
Copyright: open access
Appears in Collections:ENSP - Artigos de Periódicos
Biomanguinhos - Artigos de Periódicos
IOC - Artigos de Periódicos
MG - IRR - Artigos de Periódicos

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