Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9711
Title: Antitumour efficacy of Piper tuberculatum and piplartine based on the hollow fiber assay
Authors: Bezerra, Daniel Pereira
Ferreira, Paulo Michel Pinheiro
Machado, Camila Maria Longo
Aquino, Nayara Coryolano de
Silveira, Edilberto Rocha
Chammas, Roger
Pessoa, Claudia do Ó
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal do Piauí. Departamento de Biofísica e Fisiologia. Programa de Pós-Graduação em Ciências Farmacêuticas. Teresina, PI, Brasil
Universidade de São Paulo. Faculdade de Medicina. Departamento de Radiologia. São Paulo, SP, Brasil
Universidade Federal do Ceará. Departamento de Química Orgânica. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Departamento de Química Orgânica. Fortaleza, CE, Brasil
Universidade de São Paulo. Faculdade de Medicina. Departamento de Radiologia. São Paulo, SP, Brasil
Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia. Fortaleza, CE, Brasil / Fundação Oswaldo Cruz. Fortaleza, CE, Brasil
Abstract: Piper tuberculatum, popularly known in Brazil as “jaborandi falso” and “pimenta darta”, is widely used in folk medicine for the treatment of several diseases. In this study, the in vivo hollow fiber assay was used to investigate the antitumour efficacy of the crude extract and piplartine obtained from P. tuberculatum roots. Human glioblastoma (SF-295) and colon carcinoma (HCT-8) cell lines were used. In vitro cytotoxicity was assayed by the MTT assay. In the hollow fiber assay, nude mice implantedwith tumour cells in hollow fibers were treated for four consecutive days via the intraperitoneal route, and tumour cell populations were assessed by the MTT assay. Both the crude extract and piplartine displayed cytotoxicity. In the hollow fiber assay, tumour growth inhibition rates were 24.6–54.8% for the crude extract and 33.7–62.2% for piplartine. No signal of toxicity was noticed. In conclusion, the crude extract and piplartine obtained from P. tuberculatum roots displayed in vitro and in vivo anticancer efficacy.
Keywords: Piper tuberculatum
Piperaceae
Piplartine
Antitumour
Hollow fiber
Issue Date: 2015
Publisher: Georg Thieme Verlag
Citation: BEZERRA, D. P. et al. Antitumour efficacy of Piper tuberculatum and piplartine based on the hollow fiber assay. Planta Medica, v. 81, 15–19, 2015.
DOI: dx.doi.org/10.1055/s-0034-1383363
ISSN: 0032‑0943
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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