Objective:To analyze the presence of HPV-DNA and TIMP-2 gene methylation in cervical precursor and invasive lesions, aswell as to study the associations among the latter, the presence of HPV-DNA, and the clinical evolution of such lesions. Methods:Cross-sectional study that includes 49 biopsy or brush smear samples from women with a normal cer-vix, LSIL, HSIL, microinvasive carcinoma and invasive carcinoma. The presence of HPV-DNA and specificmethyl-ation was analyzed using PCR. Thirty-eight biopsy samples for HSIL, microinvasive carcinoma and frank invasive carcinoma as well as 11 brush smear samples for LSIL and normal cervices were analyzed. Results:TIMP-2 gene methylation was detected in 86.8% (33/38) of the samples fromthe group with lesions and 50% (4/8) of the normal samples (p= 0.03). HPV-DNA was detected in 81.6% (31/38) of the samples from the group with lesions and 25% (2/8) of the normal samples (p= 0.003). HPV-DNA wasmore frequent in the meth-ylated samples (50%), and the group with methylation had a higher risk of unfavorable evolution than the group without methylation; however, such observations were not statistically significant (p=0.19). Conclusion:TIMP-2genemethylationand thepresenceofHPV-DNAwere characteristic of thegroupwithcervical lesions. Methylation was not associated with the presence of HPV-DNA or an unfavorable clinical evolution.