Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9803
Title: Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
Authors: Marques, Sirlei Garcia
Silva, Conceição de Maria Pedroso e
Resende, Maria Aparecida
Silva, Antonio Augusto Moura da
Caldas, Arlene de Jesus Mendes
Costa, Jackson Mauricio Lopes
Affilliation: Federal University of Maranhão. Nucleus of Tropical Pathology and Social Medicine. Department of Pathology. São Luis, MA, Brasil
Federal University of Maranhão. Nucleus of Tropical Pathology and Social Medicine. Department of Pathology. São Luis, MA, Brasil
Federal University of Minas Gerais. Institute of Biological Sciences. Department of Microbiology. Belo Horizonte, MG, Brasil
Federal University of Maranhão. Department of Public Health. São Luis, MA, Brasil
Federal University of Maranhão. Nucleus of Tropical Pathology and Social Medicine. Department of Pathology. São Luis, MA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: An experimental study was conducted between January 2002 and April 2003 for the detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin) in skin tests. A total of 194 subjects were attended by spontaneous demand at the Infectious and Parasitic Diseases outpatient clinic of the Federal University of Maranhão-UFMA and at the Department of Microbiology, Federal University of Minas Gerais-UFMG and classified into three groups: patients with chromoblastomycosis caused by F. pedrosoi (n=20), healthy subjects (n=86) and patients with other diseases (n=88). For the skin test, 0.1 ml of the antigen was applied to the anterior side of the right forearm and 0.1 ml Smith medium was applied to the anterior side of the left forearm as control. The results were analyzed 48 h after inoculation of the antigen and an induration >/= 5 mm was considered to indicate a positive test. A cellular immune response to chromomycin was detected in 18 (90.0%) of the 20 patients with chromoblastomycosis caused by F. pedrosoi, and one of the patients with a negative test had reactional leprosy. Eighty-five (98.8%) of the 86 healthy subjects presented a negative reaction and only one reacted positively to the antigen. The skin test was negative in all 88 (100%) patients with other diseases, such as dermatophytosis, paracoccidioidomycosis, pulmonary aspergilloma, candidiasis, pityriasis versicolor, tuberculosis, leprosy, tegumentary leishmaniasis and syphilis, and one case of chromoblastomycosis caused by Rhinocladiella aquaspersa. Chromomycin was effective in detecting delayed hypersensitivity in patients with chromoblastomycosis caused by F. pedrosoi, with a sensitivity and specificity of 90.0% and 98.8%, respectively. These results suggest that this antigen can be used in the auxiliary diagnosis of the disease and also in epidemiological studies for determination of the prevalence of chromoblastomycosis infection in endemic areas.
Keywords: Cromoblastomycosis
Fonsecaea pedrosoi
Chromomycin
Skin test
Metabolic antigen
DeCS: Antígenos de Fungos/imunologia
Cromoblastomicose/diagnóstico
Cromomicinas/imunologia
Hipersensibilidade Tardia
Phialophora/imunologia
Testes Cutâneos/métodos
Adulto
Idoso
Antígenos de Fungos/uso diagnóstico
Cromomicinas/uso diagnóstico
Feminino
Humanos
Masculino
Meia-Idade
Sensibilidade e Especificidade
Issue Date: 2008
Publisher: Japanese Journal of Medical Mycology
Citation: MARQUES, S. G. et al. Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin). Japanese Journal of Mycology, v. 49, n. 2, p.95-101, 2008.
ISSN: 0916-4804
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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