Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9812
Title: Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis.
Authors: Machado, Anderson Silva
Carneiro, Ana Carolina Aguiar Vasconcelos
Béla, Samantha Ribeiro
Andrade, Gláucia Manzan Queiroz
Santos, Daniel Vitor Vasconcelos
Januário, José Nélio
Reis, Jordana Grazziela Alves Coelho dos
Ferro, Eloisa Amália Vieira
Carvalho, Andréa Teixeira
Vitor, Ricardo Wagner Almeida
Martins Filho, Olindo Assis
Affilliation: Universidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Pediatria. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Oftalmologia e Otorrinolaringologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Nucleo de Acoes e Pesquisa em Apoio Diagnostico. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil
Universidade Federal de Uberlandia. Uberlandia, MG, Brazil
Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil /Universidade Federal de Minas Gerais. Congenital Toxoplasmosis Brazilian Group
Abstract: Toxoplasma gondii is the main infectious cause of human posterior retinochoroiditis, the most frequent clinical manifestation of congenital toxoplasmosis. This investigation was performed after neonatal screening to identify biomarkers of immunity associated with immunopathological features of the disease by flow cytometry. The study included infected infants without NRL and with retinochoroidal lesions (ARL, ACRL, and CRL) as well as noninfected individuals (NI). Our data demonstrated that leukocytosis, with increased monocytes and lymphocytes, was a relevant hematological biomarker of ARL. Immunophenotypic analysis also revealed expansion of CD14(+)CD16(+)HLA-DR(high) monocytes and CD56(dim) cytotoxic NK-cells in ARL. Moreover, augmented TCRγ δ (+) and CD8(+) T-cell counts were apparently good biomarkers of morbidity. Biomarker network analysis revealed that complex and intricated networks underscored the negative correlation of monocytes with NK- and B-cells in NRL. The remarkable lack of connections involving B-cells and a relevant shift of NK-cell connections from B-cells toward T-cells observed in ARL were outstanding. A tightly connected biomarker network was observed in CRL, with relevant connections of NK- and CD8(+) T-cells with a broad range of cell subsets. Our findings add novel elements to the current knowledge on the innate and adaptive immune responses in congenital toxoplasmosis
Keywords: Toxoplasma gondii
Congenital Toxoplasmosis
Issue Date: 2014
Publisher: Hindawi Pub. Corp
Citation: MACHADO, Anderson Silva et al. Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. Mediators Inflamm, v. 2014, p. 910621, 2014
DOI: 10.1155/2014/910621.
ISSN: 0962-9351
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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