Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9850
Title: Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli
Authors: Stoco, Patrícia Hermes
Wagner, Glauber
Talavera-Lopez, Carlos
Gerber, Alexandra
Zaha, Arnaldo
Thompson, Claudia Elizabeth
Bartholomeu, Daniella Castanheira
Luckemeyer, Debora Denardin
Bahia, Diana
Loreto, Elgion
Prestes, Elisa Beatriz
Lima, Fabio Mitsuo
Rodrigues Luiz, Gabriela
Vallejo, Gustavo Adolfo
Silveira Filho, Jose Franco da
Schenkman, Sergio
Monteiro, Karina Mariante
Tyler, Kevin Morris
Almeida, Luiz Gonzaga Paula de
Ortiz, Mauro Freitas
Chiurillo, Miguel Angel
Moraes, Milene Hoehr de
Cunha, Oberdan de Lima
Mendonça Neto, Rondon
Silva, Rosane
Teixeira, Santuza Maria Ribeiro
Murta, Silvane Maria Fonseca
Sincero, Thais Cristine Marques
Mendes, Tiago Antonio de Oliveira
Urmenyi, Turan Peter
Silva, Viviane Grazielle
Rocha, Wanderson Duarte Da
Andersson, Bjorn
Romanha, Alvaro Jose
Steindel, Mario
Vasconcelos, Ana Tereza Ribeiro de
Grisard, Edmundo Carlos
Affilliation: Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil
Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil/Universidade do Oeste de Santa Catarina. Joaçaba, SC, Brazil
Karolinska Institutet. Science for Life Laboratory. Department of Cell and Molecular Biology. Stockholm, Sweden
Laboratorio Nacional de Computação Cientıfica. Petropolis, Rio de Janeiro, Brazil
Universidade Federal do Rio Grande do Sul. Porto Alegre, RG, Brazil
Laboratorio Nacional de Computação Cientıfica. Petropolis, Rio de Janeiro, Brazil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil
Universidade Federal de Santa Maria. Santa Maria, RG, Brazil
Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil
Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Universidad del Tolima. Ibague, Colombia
Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil
Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil
Universidade Federal do Rio Grande do Sul. Porto Alegre, RG, Brazil
Universidad Centroccidental Lisandro Alvarado. Barquisimeto, Venezuela
Laboratorio Nacional de Computação Cientıfica. Petropolis, Rio de Janeiro, Brazil
Universidade Federal do Rio Grande do Sul. Porto Alegre, RG, Brazil
Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil/ Universidad Centroccidental Lisandro Alvarado. Barquisimeto, Venezuela
Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil
Laboratorio Nacional de Computação Cientıfica. Petropolis, RJ, Brazil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brazil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil
Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brazil
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Universidade Federal do Parana. Curitiba, PR, Brazil
Karolinska Institutet. Science for Life Laboratory. Department of Cell and Molecular Biology. Stockholm, Sweden
Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil
Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil
Karolinska Institutet. Science for Life Laboratory. Department of Cell and Molecular Biology. Stockholm, Sweden
Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil
Abstract: BACKGROUND:Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts. METHODOLOGY/PRINCIPAL FINDINGS:The T. rangeli haploid genome is ∼ 24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heat-shock proteins. CONCLUSIONS/SIGNIFICANCE: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets.
Keywords: Trypanosoma rangeli
Chagas disease
Issue Date: 2015
Publisher: Public Library of Science
Citation: STOCO, Patrıcia Hermes et al. Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli. PLoS Negl Trop Dis, v. 8, n. 9, p. e3176.
DOI: Public Library of Science
ISSN: 1553-7366
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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