Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10867
Title: Pulsed-Field Gel Electrophoresis characterization of Listeria monocytogenes isolates from cheese manufacturing plants in São Paulo, Brazil
Authors: Barancelli, Giovana V
Camargo, Tarsila M
Gagliardi, Natália G
Porto, Ernani
Souza, Roberto A
Campioni, Fabio
Falcão, Juliana P
Hofer, Ernesto
Cruz, Adriano G
Oliveira, Carlos A. F
Affilliation: Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Engenharia de Alimentos. Pirassinunga, SP, Brasil.
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz. Departamento de Agroindústria, Alimentos e Nutrição. Piracicaba, SP, Brasil.
Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Engenharia de Alimentos. Pirassinunga, SP, Brasil.
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz. Departamento de Agroindústria, Alimentos e Nutrição. Piracicaba, SP, Brasil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto. SP, Brasil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto. SP, Brasil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto. SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brasil.
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Engenharia de Alimentos. Pirassinunga, SP, Brasil.
Abstract: This study aimed to evaluate the occurrence of Listeria monocytogenes in cheese and in the environment of three small-scale dairy plants (A, B, C) located in the Northern region state of São Paulo, Brazil, and to characterize the isolates using conventional serotyping and PFGE. A total of 393 samples were collected and analyzed from October 2008 to September 2009. From these, 136 came from dairy plant A, where only L. seeligeri was isolated. In dairy plant B, 136 samples were analyzed, and L. innocua, L. seeligeri and L. welshimeri were isolated together with L. monocytogenes. In dairy plant C, 121 samples were analyzed, and L. monocytogenes and L. innocua were isolated. Cheese from dairy plants B and C were contaminated with Listeria spp, with L. innocua being found in Minas frescal cheese from both dairy plants, and L. innocua and L. monocytogenes in Prato cheese from dairy plant C. A total of 85 L. monocytogenes isolates were classified in 3 serotypes: 1/2b, 1/2c, and 4b, with predominance of serotype 4b in both dairy plants. The 85 isolates found in the dairy plants were characterized by genomic macrorestriction using ApaI and AscI with Pulsed Field Gel Electrophoresis (PFGE). Macrorestriction yielded 30 different pulsotypes. The presence of indistinguishable profiles repeatedly isolated during a 12-month period indicated the persistence of L.monocytogenes in dairy plants B and C,which weremore than 100 km away from each other. Brine used in dairy plant C contained more than one L. monocytogenes lineage. The routes of contamination were identified in plants B and C, and highlighted the importance of using molecular techniques and serotyping to track L. monocytogenes sources of contamination, distribution, and routes of contamination in dairy plants, and to develop improved control strategies for L. monocytogenes in dairy plants and dairy products.
Keywords: Listeria monocytogenes
Brazil
Dairy plants
Cheese
Contamination
keywords: Brasil
DeCS: Listeria
Laticinios
Queijo
Contaminação
Issue Date: 2014
Publisher: Elsevier
Citation: BARANCELLI, Giovana V. et al. Pulsed-Field Gel Electrophoresis characterization of Listeria monocytogenes isolates from cheese manufacturing plants in São Paulo, Brazil. International Journal of Food Microbiology, v.173, n.3, p.21–29. March 2014.
DOI: 10.1016/j.ijfoodmicro.2013.12.018
ISSN: 0168-1605
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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