Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11032
Title: Mixed inflammatory/regulatory cytokine profile marked by simultaneous raise of interferon-gamma and interleukin-10 and low frequency of tumour necrosis factor-alpha(+) monocytes are hallmarks of active human visceral Leishmaniasis due to Leishmania chagasi infection.
Authors: Magalhães, Vanessa Peruhype
Martins Filho, Olindo Assis
Prata, Aluizio
Silva, L. de A
Rabello, Ana
Carvalho, Andreia Teixeira de
Figueiredo, R. M
Carvalho, Silvio Fernando Guimarães de
Ferrari, T. C. A
Van Weyenbergh, Johan Jozef Rosa Maria
Oliveira, R. Correa
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil.
Faculdade de Medicina do Triângulo Mineiro. Uberaba, MG, Brasil.
Faculdade de Medicina do Triângulo Mineiro. Uberaba, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Ouro Preto. Escola de Farmácia. Ouro Preto, MG, Brasil.
Centro Geral de Pediatria. FHEMIG, Belo Horizonte, MG, Brasil.
Universidade Estadual de Montes Claros. Montes Claros, MG, Brasil.
Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.
Abstract: Considering the complexity of the immunological events triggered during active visceral Leishmaniasis (VL), the relevance of the segregation of the immune response during human VL into type 1 and type 2 still remains unclear. For this purpose, in individuals living in risk areas for VL, we have evaluated especially asymptomatic individuals and patients with active VL, the plasmatic levels of cytokines and reactive nitrogen species under ex vivo conditions. In addition, we have also performed an analysis of intracellular cytokine patterns of circulating leucocytes after short-term culture, particularly in the absence of antigenic-specific stimulation, in order to reflect dynamic events of immune response in vivo during Leishmania chagasi infection. Although asymptomatic individuals and non-infected subjects presented a similar immunological profile, an outstanding inflammatory/regulatory profile, based on higher plasmatic levels of cytokines such as interleukin (IL)-8, interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha, IL-6 and IL-10, was associated with clinical status observed in active VL. In this context, we hypothesize that IL-10, through its ability to inhibit anti-leishmanial macrophage activation, associated with the lower frequency of TNF-alpha(+) monocytes and ordinary levels of nitrite and nitrate are the major mechanisms associated with disease onset.
Keywords: Cellular immune response
Cytometric bead array
Flow cytometry
Immunoregulation
Visceral Leishmaniasis
DeCS: Citocinas/sangue
Leishmaniose Visceral/imunologia
Monócitos/imunologia
Adolescente
Adulto
Idoso
Células Cultivadas
Criança
Pré-Escolar
Feminino
Humanos
Imunidade Celular
Interferon gama/sangue
Interleucina-10/sangue
Leishmaniose Visceral/sangue
Leucócitos Mononucleares/imunologia
Masculino
Meia-Idade
Fator de Necrose Tumoral alfa/sangue
Issue Date: 2006
Publisher: British Society for Immunology
Citation: MAGALHÃES, V. P. et al. Mixed inflammatory/regulatory cytokine profile marked by simultaneous raise of interferon-gamma and interleukin-10 and low frequency of tumour necrosis factor-alpha(+) monocytes are hallmarks of active human visceral Leishmaniasis due to Leishmania chagasi infection. Clinical and Experimental Immunology, v. 146, n. 1, p. 124-132, 2006.
DOI: 10.1111/j.1365-2249.2006.03171.x
ISSN: 0009-9104
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
MG - IRR - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

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