Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12089
Title: G1P[8] species A rotavirus over 27 years – Pre- and post-vaccination eras – in Brazil: Full genomic constellation analysis and no evidence for selection pressure by Rotarix vaccine
Authors: Silva, Marcelle Figueira Marques da
Rose, Tatiana Lundgren
Gómez, Mariela Martínez
Costa, Filipe Anibal Carvalho
Fialho, Alexandre Madi
Assis, Rosane Maria Santos de
Andrade, Juliana da Silva Ribeiro de
Volotão, Eduardo de Mello
Leite, José Paulo Gagliardi
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Abstract: Epidemiological data on species A rotavirus (RVA) infections have demonstrated the genetic diversity of strains circulating worldwide. Many G and P genotype combinations have been described over the years, varying regionally and temporally, especially in developing countries. However, the most common G and P genotype combinations identified in RVA human strains worldwide are G1P[8], G2P[4], G3P[8], G4P[8] and G9P[8]. RVA genotype G1P[8] strains are responsible for more than 50% of child infections worldwide and component of the two vaccines (Rotarix [RV1] and RotaTeq [RV5]) licensed globally. For a better understanding of the evolutionary mechanisms of this genotype in Brazil, phylogenetic analyses based on the 11 RVA genome segments (genomic constellation) from 90 G1P[8] RVA strains collected in two eras – (i) pre-vaccination with RV1 (1996–February 2006); (ii) post-vaccination (March 2006–2013) – in different Brazilian states were performed. The results showed the Wa-like genomic constellation of the Brazilian G1P[8] strains with a I1-R1-C1-M1-A1-N1-T1-E1-H1 specificity, except for two strains (rj14055-07 and ba19030-10) that belong to a I1-R1-C1-M1-A1-N1-T3-E1-H1 genomic constellation, evidencing the occurrence of reassortment (Wa-like AU-1-like) of the NSP3 gene. Reassortment events were also demonstrated between Brazilian G1P[8] strains and the RV1 vaccine strain in some genes in vaccinated and unvaccinated children. VP7 and VP8* antigenic site analysis showed that the amino acid substitutions observed in samples collected after the introduction of RV1 in Brazil were already detected in samples collected in the 1980s and 1990s, suggesting that mass Brazilian RV1 vaccination had no impact on the diversity observed inside antigenic sites for these two proteins.
Keywords: Acute gastroenteritis
Species A rotavirus
G1P[8] genomic constellation
Rotarix vaccine
Reassortment
DeCS: Gastroenterite
Rotavirus
Issue Date: 2015
Publisher: Elsevier
Citation: SILVA, Marcelle Figueira Marques da; et al. G1P[8] species A rotavirus over 27 years – Pre- and post-vaccination eras – in Brazil: Full genomic constellation analysis and no evidence for selection pressure by Rotarix vaccine. Infection, Genetics and Evolution, v.30, p.206–218, March 2015.
DOI: 10.1016/j.meegid.2014.12.030
ISSN: 1567-1348
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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