Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13057
Title: Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
Authors: Barbosa, André Victor
Cerqueira, Aloysio de Mello Figueiredo
Rusak, Leonardo Alves
Reis, Cristhiane Moura Falavina dos
Leal, Nilma Cintra
Hofer, Ernesto
Vallim, Deyse Christina
Affilliation: Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Enteropatógenos, Microbiologia Veterinária e de Alimentos. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Enteropatógenos, Microbiologia Veterinária e de Alimentos. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Abstract: Introduction: Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs). Methodology: We studied 71 strains of serotype 4b, including 25 isolates from human cases of listeriosis and 46 from meat-based foods, collected in Brazil between 1977 and 2010. The presence of ECs (I and II) markers and virulence genes (inlA, inlB, ilnC, inlJ and actA) were evaluated by PCR assay. The genetic relationship of ECs-positive strains was assessed by pulsed field gel electrophoresis. Results: ECI and ECII markers were found both in human and food strains, with 19.7% positive for the ECI marker and 40.8% for ECII. Most strains (97.2%) were positive for the virulence genes that were studied. Nevertheless, the actA gene amplicons showed two distinct sizes, with all ECI positive strains exhibiting a 105bp deletion. Pulsed field gel electrophoresis (PFGE) analysis allowed the recognition of highly related strains, particularly from two outbreaks of neonatal listeriosis in São Paulo State occurred in 1992 and 1997, both ECIIpositive; and two ECI strains from a human case (1982) and from bovine meat (2009). Conclusions: The presence of ECs among clinical samples and beef isolates of serotype 4b from some regions of Brazil highlights the need for rigorous control of production procedures. Furthermore, the association of ECII with two nosocomial outbreaks suggests its ability to spread in these settings.
Keywords: Listeria monocytogenes
Serotype 4b
Epidemic clones
Virulence
Epidemiology
DeCS: Listeria monocytogenes
Virulência
Epidemiologia
Issue Date: 2015
Publisher: Open Learning on Enteric Pathogens
Citation: BARBOSA, André Victor: et al. Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil. Journal of Infection in Developing Countries, v.9, n.9, p.962-969, 2015.
DOI: 10.3855/jidc.5639
ISSN: 1972-2680
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos
IOC - Artigos de Periódicos

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