Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14684
Title: Stage specific gene expression precedes morphological changes during Trypanosoma cruzi metacyclogenesis
Authors: Contreras, Victor T.
Morel, Carlos Medicis
Goldenberg, Samuel
Affilliation: Universidad de Carabobo. Departamento de Parasitologia y Microbiologia. Valencia, Venezuela.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: The transformation of epimastigotes to metacyclic trypomastigotes of the Trypanosoma cruzi clone Dm 28c has been studied in an in vitro system consisting of artificial triatomine urine supplemented with new born calf serum. The comparison of morphological data with gene expression products, as judged by the proteins synthesized during differentiation, has shown that stage specific gene activation precedes by far the morphological changes of differentiating cells. Immunoprecipitation of differentiating cell antigens with a trypomastigote stage specific antiserum has shown that although the morphological differentiation process takes six days to be completed, epimastigotes start to express the M 86 000 and the 78 000 trypomastigote antigens within the first 12 h of induction.
Keywords: Chagas' disease
Trypanosoma cruzi cloned strain
Gene expression
Cell differentiation
DeCS: Diferenciação Celular
Doença de Chagas
Trypanosoma cruzi
Expressão Gênica
Issue Date: 1985
Publisher: Elsevier
Citation: CONTRERAS, Victor T.; MOREL, Carlos M.; GOLDENBERG, Samuel. Stage specific gene expression precedes morphological changes during Trypanosoma cruzi metacyclogenesis. Molecular and Biochemical Parasitology, v.14, p.83-96, 1985.
ISSN: 1872-9428
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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