Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/15066
Title: Leptospira in breast tissue and milk of urban Norway rats (Rattus norvegicus)
Authors: Oliveira, Daiana Santos de
Figueira, Cláudio Pereira
Zhan, L
Pertile, Arsinoê Cristina
Pedra, Gabriel Ghizzi
Gusmão, I M
Wunder Júnior, Elsio Augusto
Rodrigues, G
Ramos, Eduardo Antonio Gonçalves
Ko, Albert Icksang
Childs, James E
Reis, Mitermayer Galvão dos
Costa, Federico
Affilliation: Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA
Ministério da Saúde. Secretaria Municipal de Saúde. Centro de Controle de Zoonoses. Salvador, BA, Brasil
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA
Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA / Universidade Federal da Bahia. UFBA. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA / Universidade Federal da Bahia. UFBA. Instituto de Saúde Coletiva. Salvador, BA, Brasil
Abstract: Leptospirosis is a zoonosis caused by bacteria of the genus Leptospira. The disease is globally distributed and a major public health concern. The Norway rat (Rattus norvegicus) is the main reservoir of the pathogen in urban slums of developing and developed countries. The potential routes of intra-specific leptospire transmission in rats are largely unknown. Herein, we identified pathogenic Leptospira spp. in breast tissue and milk of naturally infected rats. We examined kidney, breast tissue and milk from 24 lactating rats for the presence of leptospires using immunofluorescence, immunohistochemistry, polymerase chain reaction (PCR) and scanning electronic microscopy. All 24 rats had evidence for Leptospira in the kidneys, indicating chronic carriage. The majority of kidney-positive rats had detectable leptospires in milk (18, 75%) and breast tissue (16, 67%), as evidenced by immunofluorescence assay and immunohistochemistry. Four (17%) milk samples and two (8%) breast tissue samples were positive by quantitative real-time PCR. Scanning electron microscopy confirmed the presence of leptospires in breast tissue. No major pathological changes in breast tissue were found. This study, for the first time, identified leptospires in the milk and breast tissue of wild Norway rats, suggesting the possibility of milk-borne transmission of leptospirosis to neonates.
Keywords: Breastfeeding
Immunofluorescence
Immunohistochemistry
Rattus norvegicus
Scanning electronic microscopy
Vertical transmission
Issue Date: 2016
Publisher: Cambridge University Press
Citation: OLIVEIRA, D. S. Leptospira in breast tissue and milk of urban Norway rats (Rattus norvegicus). Epidemiology Infection, v. 144, n. 11, p. 2420-2429, 2016.
DOI: 10.1017/S0950268816000637
ISSN: 0950-2688
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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