Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/15204
Title: Analysis of antibody specificity against the third variable region of the envelope glycoprotein gp120 of HIV-1 in plasma from HIV-1-positive individuals residing in Brazil
Authors: Bongertz, Vera
Jansson, M
Flodby, P
Morgado, Mariza Gonçalves
Castro Filho, Bernardo Galvão
Wigzell, H
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Karolinska Institute. Department of Immunology. Stockholm, Sweden
Karolinska Institute. Department of Immunology. Stockholm, Sweden
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Karolinska Institute. Department of Immunology. Stockholm, Sweden
Abstract: 1. Antibody specificity for the principal neutralization domain (PND) of the human immunodeficiency virus type 1 (HIV-1) was studied in plasma from 122 HIV-1-infected individuals residing in Brazil. 2. Using 8 overlapping sequential pentadecapeptides corresponding to the third variable region (V3) of 5 different HIV-1 isolates in an enzyme-linked immunosorbent assay (ELISA), a preferential recognition of the peptides with amino acid sequences corresponding to the HIV-1 isolates IIIB and MN (maximal reactivities of 60-70%) compared to the isolates SC, WMJ-2 or RF (maximal reactivities below 60%) was observed. 3. A difference was observed in the overall reactivity pattern to HIV-1 SC peptides of plasma collected from individuals residing in the Brazilian states of Rio de Janeiro and Bahia. However, a statistically significant increased recognition by Bahian plasma was only observed for the HIV-1 SC C55 peptide. 4. The mean CD4/CD8 ratio of the group of plasma with an isolate-restricted recognition of peptides (0.522 +/- 0.074) was significantly lower than that of the total group of plasma (1.00 +/- 0.18).
Keywords: HIV-1
AIDS
Antibodies
PND
Y3 Loop
Brazil
Humans
Molecular Sequence Data
Peptide fragments
Issue Date: 1994
Publisher: Associação Brasileira de Divulgação Científica
Citation: BONGERTZ, V. et al. Analysis of antibody specificity against the third variable region of the envelope glycoprotein gp120 of HIV-1 in plasma from HIV-1-positive individuals residing in Brazil. Brazilian Journal of Medical Biological Researach, v. 27, n. 5, p. 1225-1236, 1994.
ISSN: 0100-879X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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