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A FRAMEWORK TO IDENTIFY GENE EXPRESSION PROFILES IN A MODEL OF INFLAMMATION INDUCED BY LIPOPOLYSACCHARIDE AFTER TREATMENT WITH THALIDOMIDE
Paiva, Renata T. et al. | Date Issued: 2012
Author
Paiva, Renata T.
Saliba, Alessandra M.
Fulco, Tatiana O.
Sales, Jorgenilce de Souza
Carvalho, Daniel Serra de
Sampaio, Elizabeth P.
Lopes, Ulisses G.
Sarno, Euzenir N.
Nobre, Flavio F.
Affilliation
Universidade Federal do Rio de Janeiro. Programa de Engenharia Biomédica. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Biologia Molecular e Estrutural. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Programa de Engenharia Biomédica. Rio de Janeiro, RJ, Brasil.
Abstract
Thalidomide is an anti-inflammatory and anti-angiogenic drug currently used for the treatment of several diseases, including erythema nodosum leprosum, which occurs in patients with lepromatous leprosy. In this research, we use DNA microarray analysis to identify the impact of thalidomide on gene expression responses in human cells after lipopolysaccharide (LPS) stimulation. We employed a two-stage framework. Initially, we identified 1584 altered genes in response to LPS. Modulation of this set of genes was then analyzed in the LPS stimulated cells treated with thalidomide.
Keywords in Portuguese
Talidomida
Modelo de Inflamação
Expressão genética
 
Keywords
Thalidomide
Microarray
Rank product
Inflammation model
Lipopolysaccharide
 
DeCS
Análise em Microsséries
Antígenos CD14
 
Publisher
BioMed Central
Citation
PAIVA, Renata T. et al. A framework to identify gene expression profiles in a model of inflammation induced by lipopolysaccharide after treatment with thalidomide. BMC Research Notes, v.5, n.292, 12p, 2012.
DOI
10.1186/1756-0500-5-292
ISSN
1756-0500