Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/17992
Title: Natural Inhibitors of Snake Venom Metalloendopeptidases: History and Current Challenges
Authors: Bastos, Viviane A.
Gomes Neto, Francisco
Perales, Jonas
Ferreira, Ana Gisele C. Neves
Valente, Richard H.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas, CNPq. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas, CNPq. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas, CNPq. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas, CNPq. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas, CNPq. Brasília, DF, Brasil.
Abstract: The research on natural snake venom metalloendopeptidase inhibitors (SVMPIs) began in the 18th century with the pioneering work of Fontana on the resistance that vipers exhibited to their own venom. During the past 40 years, SVMPIs have been isolated mainly from the sera of resistant animals, and characterized to different extents. They are acidic oligomeric glycoproteins that remain biologically active over a wide range of pH and temperature values. Based on primary structure determination, mammalian plasmatic SVMPIs are classified as members of the immunoglobulin (Ig) supergene protein family, while the one isolated from muscle belongs to the ficolin/opsonin P35 family. On the other hand, SVMPIs from snake plasma have been placed in the cystatin superfamily. These natural antitoxins constitute the first line of defense against snake venoms, inhibiting the catalytic activities of snake venom metalloendopeptidases through the establishment of high-affinity, non-covalent interactions. This review presents a historical account of the field of natural resistance, summarizing its main discoveries and current challenges, which are mostly related to the limitations that preclude three-dimensional structural determinations of these inhibitors using "gold-standard" methods; perspectives on how to circumvent such limitations are presented. Potential applications of these SVMPIs in medicine are also highlighted.
Keywords: cross-linking; hydrogen/deuterium exchange
mass spectrometry
metalloendopeptidase inhibitor
modeling
natural immunity
natural resistance
snake venom
structure
therapeutic application
keywords: Modelagem
Espectrometria de Massas
Imunidade natural
Inibidor da metalloendopeptidase
Resistência natural
veneno de cobra
Issue Date: 2016
Publisher: MDPI
Citation: BASTOS, Viviane A. et al. Natural Inhibitors of Snake Venom Metalloendopeptidases: History and Current Challenges. Toxins, v.8, n.9, 250, 20p, 2016.
DOI: 10.3390/toxins8090250
ISSN: 2072-6651
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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