Biotechnology is a millenary science, however, was in the last 60 years which reached its “peak/top” with the advances in the techniques for manipulating living beings. About your multidisciplinary nature, in the countries where it was considered a strategic element for technological innovation, many systems of organization have been developed for their own development. In order to analyze preliminary organizational factors of developing a National Innovation System for Biotechnology in Venezuela, we conducted this study using interviews and electronic questionnaires with different actors in the system of R&D: universities researchers, funding agencies, representatives of government and companies. This paper suggests some strength such as Venezuelan tradition in Biotechnology and high level of human capital qualification. Biotechnology in Venezuela was mainly developed in public research institutions such as universities and government centers, with two priority areas: agriculture and life science. However, there is a long way forward before Biotechnology is incorporated in the desired economic and social development, such as: the development of mechanisms for continued government funding and venture capital to create start-ups enterprises, strengthening strategies links between universities and companies and networking, and the development of a specific legislation for Biotechnology.