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MITOCHONDRIAL GENOME INSTABILITY IN COLORECTAL ADENOMA AND ADENOCARCINOMA
Araujo, Luiza Ferreira de et al. | Date Issued: 2015
Author
Araujo, Luiza Ferreira de
Fonseca, Aline Simonete
Muys, Bruna Rodrigues
Plaça, Jessica Rodrigues
Bueno, Rafaela de Barros e Lima
Lorenzi, Julio Cesar Cetrulo
Santos, Anemari Ramos Dinarte dos
Molfetta, Greice Andreotti de
Zanette, Dalila Lucíola
Souza, Jorge Estefano Santana de
Valente, Valeria
Silva Junior, Wilson Araujo da
Affilliation
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil
Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil
Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil / Center for Medical Genomics. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil / Center for Medical Genomics. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil
Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil / Center for Medical Genomics. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil
Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil / Center for Medical Genomics. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil / University of São Paulo State. Faculty of Pharmaceutical Science of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Center for Cell-Based. Therapy Regional Blood Center of Ribeirão Preto. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil / Center for Medical Genomics. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil
Abstract
Mitochondrial dysfunction is regarded as a hallmark of cancer progression. In the current study, we evaluated mitochondrial genome instability and copy number in colorectal cancer using Next Generation Sequencing approach and qPCR, respectively. The results revealed higher levels of heteroplasmy and depletion of the relative mtDNA copy number in colorectal adenocarcinoma. Adenocarcinoma samples also presented an increased number of mutations in nuclear genes encoding proteins which functions are related with mitochondria fusion, fission and localization. Moreover, we found a set of mitochondrial and nuclear genes, which cooperate in the same mitochondrial function simultaneously mutated in adenocarcinoma. In summary, these results support an important role for mitochondrial function and genomic instability in colorectal tumorigenesis.
Keywords in Portuguese
Genoma mitocondrial
Câncer colorretal
Heteroplasmia
Instabilidade do genoma
 
Keywords
Mitochondrial genome
Colorectal cancer
Heteroplasmy
Genome instability
 
Publisher
Springer Verlag
Citation
ARAUJO, L. F. et al. Mitochondrial genome instability in colorectal adenoma and adenocarcinoma. Tumor Biology, v. 36, n. 11, p. 8869-8879, 2015.
DOI
10.1007/s13277-015-3640-7
ISSN
1010-4283
Notes
Zanette, Dalila Lucíola “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.