Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/21003
Title: Identification of a major Quantitative Trait Locus determining resistance to the organophosphate temephos in the dengue vector mosquito Aedes aegypti
Authors: Paiva, Marcelo H. S.
Lovin, Diane D.
Mori, Akio
Melo-Santos, Maria A. V.
Severson, David W.
Ayres, Constância F. J.
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil
Abstract: Organophosphate insecticides (OP) have extensively been used to control mosquitoes, such as the vector Aedes aegypti. Unfortunately, OP resistance has hampered control programs worldwide. We used Quantitative Trait Locus (QTL) mapping to evaluate temephos resistance in two F1 intercross populations derived from crosses between a resistant Ae. aegypti strain (RecR) and two susceptible strains (MoyoD and Red). A single major effect QTL was identified on chromosome 2 of both segregating populations, named rtt1 (resistance to temephos 1). Bioinformatics analyses identified a cluster of carboxylesterase genes (CCE) within the rtt1 interval. qRT-PCR demonstrated that different CCEs were up-regulated in F2 resistant individuals from both crosses. However, none exceeded the 2-fold expression. Primary mechanisms for temephos resistance may vary between Ae. aegypti populations, yet also appear to support previous findings suggesting that multiple linked esterase genes may contribute to temephos resistance in the RecR strain as well as other populations.
Keywords: Aedes
drug effects
genetics
Animals
Chromosomes, Insect
Insecticide Resistance
Insecticides
Quantitative Trait Loci
Temefos
toxicity
keywords: Aedes
Resistência a Inseticidas
Locos de Características Quantitativas
DeCS: Aedes
Animais
Cromossomos de Insetos
Resistência a Inseticidas
Inseticidas
Locos de Características Quantitativas
Temefós
Issue Date: 2016
Citation: PAIVA, M. H. S. et al. Identification of a major Quantitative Trait Locus determining resistance to the organophosphate temephos in the dengue vector mosquito Aedes aegypti. Genomics, v. 107, n. 1, p. 40–48, jan. 2016.
ISSN: 1089-8646
Copyright: open access
Appears in Collections:IAM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
26576515 2015 pai-ana.oa.pdf588.39 kBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.