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RAPID TESTS AND THE DIAGNOSIS OF VISCERAL LEISHMANIASIS AND HUMAN IMMUNODEFICIENCY VIRUS/ACQUIRED IMMUNODEFICIENCY SYNDROME COINFECTION
Agglutination Tests
Antigens, Protozoan
Biomarkers
Coinfection
Female
HIV Infections
Humans
Immunochromatography
Latex Fixation Tests
Leishmaniasis, Visceral
Male
Middle Aged
Protozoan Proteins
Reproducibility of Results
Sensitivity and Specificity
Young Adult
complications
diagnosis
methods
Leishmaniose Visceral
Adulto
Humanos
Feminino
Masculino
Testes de Aglutinação
Biomarcadores
Coinfecção
Infecções por HIV
Imunocromatografia
Testes de Fixação do Látex
Proteínas de protozoários
Meia-Idade
Reprodutibilidade dos testes
Sensibilidade e Especificidade
Adulto Jovem
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil
Abstract
After the emergence of the human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS), the number of visceral leishmaniasis (VL)-HIV/AIDS coinfections has increased worldwide. Herein, we assessed the usefulness of an rK39-based immunochromatographic test (rK39 ICT) (DiaMed-IT LEISH(®); DiaMed AG, Cressier-sur-Morat, Switzerland) and a latex agglutination test (KAtex; Kalon Biological, Guildford, United Kingdom) for urinary antigen detection to diagnose VL in 15 HIV/AIDS patients from northeastern Brazil. VL diagnosis was based on clinical findings, cytology, serology, parasite DNA, and/or urinary antigen detection. VL was confirmed in seven out of 15 HIV/AIDS patients. Only three patients were positive in bone marrow cytology, three patients were conventional polymerase chain reaction (PCR) positive, while six were real-time PCR positive. All patients were direct agglutination test (DAT) (Royal Tropical Institute, Amsterdam, The Netherlands) positive; of these, four were positive by rK39 ICT and five by KAtex. Large-scale studies are needed to validate the use of the KAtex in the national public health laboratory network in Brazil, aiming at improving the diagnosis of VL in HIV/AIDS patients in this country.
Keywords
AdultAgglutination Tests
Antigens, Protozoan
Biomarkers
Coinfection
Female
HIV Infections
Humans
Immunochromatography
Latex Fixation Tests
Leishmaniasis, Visceral
Male
Middle Aged
Protozoan Proteins
Reproducibility of Results
Sensitivity and Specificity
Young Adult
complications
diagnosis
methods
DeCS
Infecções por HIVLeishmaniose Visceral
Adulto
Humanos
Feminino
Masculino
Testes de Aglutinação
Biomarcadores
Coinfecção
Infecções por HIV
Imunocromatografia
Testes de Fixação do Látex
Proteínas de protozoários
Meia-Idade
Reprodutibilidade dos testes
Sensibilidade e Especificidade
Adulto Jovem
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